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Position: Home > Articles > Regulation of Unfolded Protein Response in Hep3B Cells by HCV NS4B Acta Agriculturae Universitatis Jiangxiensis 2012,34 (1) 171-175

丙型肝炎病毒NS4B蛋白调控Hep3B细胞非折叠蛋白质反应研究

作  者:
张艳妮;张庆华;陈瑶;刘好桔;刘志文;孔令保
单  位:
江西农业大学生物科学与工程学院
关键词:
丙型肝炎病毒;NS4B;Hep3B;非折叠蛋白质反应
摘  要:
研究表达的丙型肝炎病毒(HCV)NS4B对Hep3B细胞非折叠蛋白质反应的影响。NS4B重组真核表达质粒pcDNA3.1(-)NS4B通过脂质体转染Hep3B细胞,G418筛选和Western blot鉴定稳定转染细胞;RT-PCR检测稳定转染细胞内XBP1 mRNA剪接,Western blot鉴定ATF6蛋白切割,荧光素酶试验检测稳定转染细胞内GRP78和XBP1启动子活性。G418筛选和Western Blot鉴定证实获得稳定表达NS4B的Hep3B细胞;在该细胞内,XBP1 mRNA剪接、ATF6切割、XBP1和Grp78启动子激活均被检测到。NS4B在Hep3B的稳定表达诱导了非折叠蛋白质反应。
译  名:
Regulation of Unfolded Protein Response in Hep3B Cells by HCV NS4B
作  者:
ZHANG Yan-ni,ZHANG Qing-hua,CHENG Yao, LIU Hao-ju,LIU Zhi-wen,KONG Ling-bao*(College of Bioscience and Engineering,JAU,Nanchang 330045,China)
关键词:
HCV;NS4B;Hep3B;unfolded protein response
摘  要:
To study the effect of hepatitis C virus NS4B on unfolded protein response in Hep3B cells.The recombinant vector pcDNA3.1(-) NS4B with NS4B gene was transfected to Hep3B cells using Lipofectamine 2000.Stably transfected cells were selected by G418 and then confirmed by Western blot analysis;XBP1 mRNA splicing,ATF6 cleavage,GRP78 and XBP1 promoter activation were analyzed in stably transfected Hep3B cells using RT-PCR,Western blot or luciferase assays.G418 seclection and Western blot demonstrated that Hep3B cells stably expressing NS4B were obtained successfully;XBP1 mRNA splicing,ATF6 cleavage,GRP78 and XBP1 promoter activation were observed in Hep3B cells stably expressing NS4B.Stable expression of NS4B in Hep3B cells induces unfolded protein response.

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