当前位置: 首页 > 文章 > 热休克蛋白增加运动发酵单胞菌乙醇产量的研究 现代农业科学 2009 (4) 33-36+77
Position: Home > Articles > Adaptation Mediated by Hsp can Improve the Resistance to Adversity and Ethanol Production of Zymomonas mobilis Modern Agricultural Sciences 2009 (4) 33-36+77

热休克蛋白增加运动发酵单胞菌乙醇产量的研究

作  者:
李海燕;王茜;苏志国;熊平;汪浩勇
单  位:
湖北工业大学湖北省工业微生物重点实验室;中国科学院过程工程研究所生化工程国家重点实验室
关键词:
逆境适应;运动发酵单胞菌;Hsp;高糖;乙醇产量
摘  要:
目的:对含有Hsp基因的代谢工程运动发酵单胞菌进行热适应处理,用于提高其乙醇产量和对高糖的耐受性。方法:用含有Hsp基因的多顺反子质粒转化运动发酵单胞菌,经过多天37℃热适应后,在玉米水解液中测定该多顺反子对运动发酵单胞菌发酵产生的影响。结果:在37℃发酵条件下,在糖浓度为28%的玉米水解液中,与对照组相比,经过热适应的含Hsp基因的代谢工程菌的乙醇产量显著提高。结论:Hsp基因能够显著提高运动发酵单胞菌的乙醇产量。
译  名:
Adaptation Mediated by Hsp can Improve the Resistance to Adversity and Ethanol Production of Zymomonas mobilis
作  者:
LI Haiyan 1, WANG Xi1, SU ZhiGuo 2,XIONG Ping1, WANG Haoyong 1,2, (1 Hubei University of Technology, Wuhan,430068;2 Institute of Process Engineering Chinese Academy of Sciences,Beijing, 100190, China)
关键词:
adversity adaptation; Zymomonas mobilis; Hsp; high sugar concentration; ethanol production
摘  要:
Objective: Hsp was used to mediated the adversity adaptation of Zymomonas mobilis to increase it's resistance to the adversity and ethanol production. Method: Zymomonas mobilis was transformed by plasmid containing Hsp gene, then incubated at 37℃ for adversity adaptation. After days of treatment, the performance of ethanol production of the adapted strain was analyzed in corn hydrolysis media. Result: After adaptation, the production of ethanol by the Zymomonas mobilis with Hsp was 1.55 times more than it's wild type counterpart in corn hydrolysis with sugar concentration as 28% at 37℃.Conclusion: Hsp gene can significantly increase ethanol production of Zymomonas mobilis at high temperature and high sugar concentration.

相似文章

计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊