Position: Home > Articles > Effect of 5-aza-dc on the Biological Characteristics of Yanbian Dairy Goat Ear Fibroblast Cell
Hubei Agricultural Sciences
2013,52
(12)
2862-2865，2874
5-aza-dc对延边奶山羊耳成纤维细胞生物学特性的影响
作 者：
蔡丽娟;尹多;庄利利;方南洙;李钟淑
单 位：
延边大学农学院动物遗传育种与繁殖实验室
关键词：
5-aza-dc;延边奶山羊;细胞活率;细胞形态;细胞凋亡
摘 要：
通过DNA甲基转移酶抑制剂5-aza-dc对延边奶山羊耳成纤维细胞进行不同处理后,利用MTT比色法选定5-aza-dc的最适作用浓度和时间,用Hochest33342/PI双染和琼脂糖凝胶电泳检测细胞凋亡情况,通过核型分析检测5-aza-dc对细胞染色体的影响。结果表明,低浓度的5-aza-dc(≤0.010μmol/L)作用72 h对细胞影响不大;随其浓度的增加,细胞形态、活率、凋亡及染色体都受到显著影响。5-aza-dc对细胞的最佳处理时间为72 h,此时低浓度的5-aza-dc并未引起细胞凋亡和核型改变。
译 名：
Effect of 5-aza-dc on the Biological Characteristics of Yanbian Dairy Goat Ear Fibroblast Cell
作 者：
CAI Li-juan,YIN Duo,ZHUANG Li-li,FNAG Nan-zhu,LI Zhong-shu(Animal Genetic and Breeding Reproduction Laboratory,Agricultural College,Yanbian University,Yanji 133002,Jilin,China)
关键词：
5-aza-dc;Yanbian dairy goat;cell morphology;cell viability;cell apoptosis
摘 要：
To research the effects of DNA methylation inhibitor 5-aza-dc on biological characteristics of Yanbian dairy goat ear fibroblast cell,MTT method was used to determine the optimal action concentration and time of 5-aza-dc on Yanbian dairy goat ear fibroblast cells.PI and Hochest33342 double staining,agarose gel electrophoresis were used to detect apoptosis of the cells;and karyotype analysis to measure the effect of 5-aza-dc on the chromosomes.The results showed low concentration(≤0.010 μmol/L) of 5-aza-dC treatment for 72 h had little effect on the cells.With the increasing of concentration,cell morphology,viability,apoptosis and chromosomes were all significantly affected.The best processing time of 5-aza-dc was 72 h,low concentration of 5-aza-dc did not lead to apoptosis and karyotype change.
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