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当前位置: 首页 > 文章 > 卵圆型软条白沙枇杷变异种质鉴定研究 植物遗传资源学报 2014,15 (5) 986-991
Position: Home > Articles > Identification of Egg-shaped Ruantiao Baisha Loquat Mutant Germplasm Journal of Plant Genetic Resources 2014,15 (5) 986-991

卵圆型软条白沙枇杷变异种质鉴定研究

作  者：
陈方永;谢丽雪;倪海枝;王引;洪婷;任正初
单  位：
福建省农业科学院果树研究所;浙江省柑橘研究所;浙江省台州市路桥区林特总站
关键词：
白肉枇杷;SRAP;遗传多样性;亲缘关系
摘  要：
本研究以卵圆型软条白沙枇杷变异种质为试材,母株软条白沙及中国、日本、新西兰3个国家的30个白肉枇杷栽培品种为对照,以形态学性状调查为基础,结合SRAP分子标记对变异种质进行鉴定研究。从96对引物中筛选出18对进行扩增,共获得135个位点,其中102个多态性位点,多态性位点百分率为75.6%。不同品种间的遗传相似系数GS在0.53~0.89之间。在GS为0.66处,可将31份枇杷种质分为4类。第Ⅰ类为来自中国浙江的8份种质。第Ⅱ类为来自日本、新西兰和中国江苏和福建的19个种质。第Ⅲ类为上海白沙和广西白蜜枇杷2个种质。第Ⅳ类为福建白梨和龙才白2个种质。而卵圆型软条白沙与对照品种遗传相似系数为0.55~0.88,与白荔枝的遗传相似度最大(GS=0.88),与乌躬白遗传相似度最小(GS=0.55),与母株软条白沙的遗传相似系数仅为0.73,二者的遗传相似度不大。生物学性状调查表明,卵圆型软条白沙变异材料与母株软条白沙在果型、种子数和可食率等性状上具有明显差异。综合研究结果显示,卵圆形软条白沙与母株软条白沙相比,综合性状发生了明显的遗传变异,应属芽变种质材料。本研究对枇杷变异材料的创新利用、新品种审定与知识产权保护具有重要的实际意义。
译  名：
Identification of Egg-shaped Ruantiao Baisha Loquat Mutant Germplasm
作  者：
CHEN Fang-yong;XIE Li-xue;NI Hai-zhi;WANG Yin;HONG Ting;REN Zheng-chu;Citrus Institute of Zhejiang Province;Fruit Research Institute,Fujian Academy of Agricultural Sciences;Forest and Special Plant Station of Luqiao District,Taizhou City in Zhejiang Province;
关键词：
white pulp loquat;;SRAP;;genetic diversity;;genetic relationship
摘  要：
Egg-shaped ruantiao baisha mutant and 30 loquat germplasms from China,Japan and New Zealand were used as plant material and contrast,respectively in this paper. Identification of the mutant germplasm were constructed with SRAP molecular marker based on the morphological investigation. 18 pairs of primers were selected from 96 pairs of SRAP primers. Finally,135 loci were detected,of which 102 were polymorphic and the polymorphic percentage was 75. 6%. The genetic similarity ranged from 0. 53 ~ 0. 89. 31 white pulp loquat germplasms were divided into four groups at the similar coefficient of 0. 65. The loquat germplasms came from Zhejiang province were clustered together into the first group. The second group contained 19 germplasms which came from China,Japan,and New Zealand. The third group included Shanghai baisha and Guangxin bami. The fourth group included Bailizhi and Longcai bai. The genetic simlity between egg-shaped ruantiao baisha and the other contrasted germplasms ranged from 0. 55 to 0. 88. It had the maximum GS with Bailizhi( GS = 0. 88),and minimun GS with Wugongbai( GS= 0. 55),while the GS between egg-shaped ruantiao baisha and ruantiao baisha was only 0. 73. Study of biological characters showed that egg-shaped ruantiao baisha and ruantiao baisha had significant differences among fruit shape,seed number,and edible rate. As the comprehensive results,the egg-shaped ruantiao baisha had significant genetic variation with ruantiao baisha loquat,and it should be a mutant germplasm. The study would be of great practical significance to new varieties innovation and protection of intellectual property rights for loquat mutantgermplasm.

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