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Position: Home > Articles > TWO STEP FREEZING OF COW EMBRYOS Chinese Journal of Animal and Veterinary Sciences 1984,15 (3) 1-4

牛胚胎两步冷冻

作  者:
朱裕鼎;罗应荣;郭年藩;冯书堂;蒋世娥;朱成宽
摘  要:
8枚61/2~71/2日龄黑白花奶牛胚胎,采用两步冷冻和细管包装法进行了冷冻。同时配合使用甘油抗冻剂,即以1℃/分钟的速度由室温降至-5℃;在-5℃下诱发结晶并在投入液氮前以0.3℃/分钟的速度降温至-36℃。胚胎在液氮中贮存4天~7个月后,直接在17℃温水中解冻。除去甘油后,用评定胚胎形态的方法进行胚胎存活的鉴定,6枚形态评为优和良的胚胎,用凯苏式输卵器非手术分别移入6头同期受体母牛。其中2头妊娠,1头妊娠母牛在孕后第四个月流产,另1头于1983年元月26日产1公犊,犊牛初生重为45公斤。试验结果证明,采用两步冷冻法冷冻的牛胚胎移植后可以成活。
译  名:
TWO STEP FREEZING OF COW EMBRYOS
作  者:
Zhu Yuding Luo Yingrong Guo Nianfan Feng Shutang Zhu Chengkuan Jiang Shie (Institute of Animal Sciences , Chinese Academy of Agricultural Sciences Beijing,China)
摘  要:
Eight cow embryos of black and white breed collected at 61/2~71/2 day of oestrum cycle were frozen in straws by two step freezing,in whi- ch were included cooling at 1℃/min from room temperature to -5℃, seeding at -5℃ and freezing at 0.3℃/min to -36℃ before being plunged into liquid nitrogen,at the same time,the glycerol as a cryoprotectant was used.After 4 days……7 monthes storage in the liquid nitrogen the embryos were thawed by direct transfer to water at 17℃. Embryo survival was assessed by morphological evaluation after gly- cerol dilution and 6 morphologically exeollent or good embryos were tra- sfered non-surgically to 6 recipient cows with a Casson insemination gun respectively.Two of them were pregnant.One recipicent at the fourth month of pregnant aborted,and another produced a male calf on January 26,1983.The calf produced from transfer of frozen embryoshad a birth-weight of 45 kg. The experiment demonstrated that frozen embryos by two step free- zing can be able to survive after transfer.

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