作 者：
于鑫;唐敏敏;罗家欣;宋菲;朱婷婷;陈华;赵松林
关键词：
槟榔提取物;血管紧张素转化酶;抑制作用;酶动力学
摘 要：
以嫩果期槟榔籽为研究对象,采用 75%乙醇浸提制得槟榔籽提取物(areca nut extract,ANE),再分别用石油醚、乙酸乙酯和正丁醇依次对总提取物进行萃取,依次得到嫩果期槟榔籽 75%乙醇提取物的石油醚部位(PE-ANE)、乙酸乙酯部位(EAC-ANE)、正丁醇部位(BU-ANE)、水部位(W-ANE)的萃取部分.通过体外抑制血管紧张素转化酶(ACE)活性抑制试验测定槟榔提取物各极性部位对高血压的抑制潜力;通过酶动力学实验分析ACE抑制活性最好的极性部位对 ACE 活性的抑制作用类型;测定不同极性部位蛋白质、总糖及多酚的总含量,并分析其与 ACE 半抑制浓度(IC50)之间的相关性;最后对 4 种不同极性部位萃取物中的多酚组分进行初步定量.结果表明:嫩果期槟榔籽提取物各极性部位均具有一定的ACE抑制活性,其中EAC-ANE的ACE抑制活性最好.酶动力学实验表明,EAC-ANE对 ACE活性的抑制作用类型为竞争与非竞争的混合型抑制.相关性分析表明,各极性部位 ACE的 IC50 值与多酚、总糖及蛋白质含量均呈显著负相关,且与多酚含量之间具有极强相关性(r=-0.912).在 4 个极性部位中定量了儿茶素、L-表儿茶素、原花青素B2、原儿茶酸、槲皮素等多种多酚组分,且EAC-ANE中的相关多酚组分含量最高.综上所述,槟榔提取物具有一定的降血压潜力,且乙酸乙酯萃取部位最好,多酚含量与各萃取部位的ACE抑制活性具有极强的相关性,可初步推断多酚是槟榔提取物中抑制ACE活性的重要物质.
译 名：
Inhibitory Effects of Areca Nut Extract on the Activity of Angiotensin Converting Enzyme
关键词：
areca nut extract%angiotensin converting enzyme(ACE)%inhibitory activity%enzyme kinetics
摘 要：
In this study,75%ethanol solution was used to prepare the total extracts of arecanut nut(ANE).Then differ-ent fractions of the extracts were prepared by fractionating with petroleum ether(PE-ANE),ethyl acetate(EAC-ANE),n-butanol(BU-ANE)and water(W-ANE)in turn.The inhibition of hypertension from ANE was determined by angio-tensin converting enzyme(ACE)activity inhibition assay in vitro.The inhibitory was evaluated with the fraction dis-playingthe best ACE inhibitory activity by the enzyme kinetic method.In addition,the contents of total polyphenols,total sugar and total protein in each fraction of ANE were determined.The correlation between the contents of the compositions and ACE inhibitory activities was analyzed.Finally,phenoliccomponents in the four fractions were ana-lyzed.The results showed that all the fractions of ANE at different polar parts exhibited ACE inhibitory activity,and EAC-ANE displayed the best.Enzyme kinetics tests showed that the inhibitory effect of EAC-ANE from tender areca on ACE was a mixed type of competitive inhibition and non-competitive inhibition.The correlation analysis showed that the IC50 value of the ACE inhibitory activity of each polar part of ANE was significantly negatively correlated with the content of polyphenols and total sugar.And the absolute value of the correlation coefficient between the IC50 value of ACE inhibitory activity and the polyphenol content reached a very strong correlation(r=-0.912).And a variety of polyphenol components such as catechin,L-epicatechin,procyanidin B2,protocatechuic acid,and quercetin were identi-fied in the four different polar parts,and the content of polyphenol components in EAC-ANE was the highest.In con-clusion,ANE has a certain blood pressure lowering potential,and the ethyl acetate extraction site is the best,and the polyphenol content has a strong correlation with the ACE inhibitory activity of each extract.It can be preliminarily in-ferred that polyphenols may be the main substances that inhibit the activity of ACE in ANE.
