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当前位置: 首页 > 文章 > 饲粮铜含量对牦牛体外瘤胃发酵的影响 动物营养学报 2016,28 (8) 2599-2606
Position: Home > Articles > Effects of Dietary Copper Content on Rumen Fermentation of Yaks in Vitro Chinese Journal of Animal Nutrition 2016,28 (8) 2599-2606

饲粮铜含量对牦牛体外瘤胃发酵的影响

作  者：
薛艳锋;郝力壮;刘书杰;柴沙驼;张晓卫;赵索南
单  位：
省部共建三江源生态与高原农牧业国家重点实验室;海北州畜牧兽医科学研究所
关键词：
牦牛;甘氨酸铜;体外产气技术;消化酶活力;挥发性脂肪酸
摘  要：
为了探究牦牛饲粮中微量元素铜的适宜含量,本试验以甘氨酸铜作为添加形式,以牦牛饲粮为底物进行体外瘤胃发酵,底物铜含量分别为5.0、10.0、15.0、20.0、25.0 mg/kg,共发酵48 h。测定发酵后的产气量、瘤胃发酵指标及消化酶活力。结果表明:当底物铜含量为15.0 mg/kg时,干物质消化率(DM D),微生物蛋白质(M CP)、丙酸、异丁酸、丁酸、异戊酸、戊酸、总挥发性脂肪酸浓度,脂肪酶(LPS)、胰蛋白酶(TYS)、纤维素酶(CLS)活力均达到最大值,分别为63.858%、4.289 g/L、24.475 mmol/L、0.470 mmol/L、8.977 mmol/L、1.159 mmol/L、1.607 mmol/L、81.583 mmol/L、0.504 U/m L、84.167 U/m L、79.956 U/m L,乙酸/丙酸最低为2.045;当铜含量为10.0 mg/kg时,M CP、乙酸浓度达到最大值,分别为4.289 g/L、51.075 mmol/L,且其他指标也都处于较高水平。综合得出,在体外条件下,牦牛饲粮推荐铜含量为10.0~15.0 mg/kg。
译  名：
Effects of Dietary Copper Content on Rumen Fermentation of Yaks in Vitro
作  者：
XUE Yanfeng;HAO Lizhuang;LIU Shujie;CHAI Shatuo;ZHANG Xiaowei;ZHAO Suonan;State Key Laboratory of Plateau Ecology and Agriculture,Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province,Qinghai Plateau Yak Research Center,Qinghai Academy of Science and Veterinary Medicine of Qinghai University;Academy of Science and Veterinary Medicine of Haibei Prefecture;
关键词：
yak;;cupric glycinate;;in vitro gas production technique;;digestive enzyme activity;;volatile fatty acid
摘  要：
To find an optimal content of copper in diet for yaks,cupric glycinate was used as additive,and diet for yaks was used as a substrate for in vitro rumen fermentation in the present study.Copper contents in substrates were designed as 5.0,10.0,15.0,20.0,25.0 mg/kg,respectively.The fermentation lasted for 48 h.After fermentation,gas production,rumen fermentation parameters and digestive enzyme activities were measured.The results showed as follows:when the content of copper in substrate was 15.0 mg/kg,dry matter digestibility(DM D),the concentrations of microbial protein(M CP),propionic acid,isobutyric acid,butyric acid,isovaloric acid,valeric acid and total volatile fatty acids,as well as the activities of lipidase,trypsinase,cellulase reached the highest,which were 63.858 %,4.289 g/L,24.475 mmol/L,0.470 mmol/L,8.977 mmol/L,1.159 mmol/L,1.607 mmol/L,81.583 mmol/L,0.504 U/m L,84.167 U/m L and79.956 U/m L,respectively;when the content of copper in substrate was 10.0 mg/kg,acetic acid/propionic acid reached the lowest,which was 2.045;when the content of copper was 10.0 mg/kg,M CP and acetic acid concentrations reached the highest,which were 4.289 g/L and 51.075 mmol/L,respectively,and the values of other indexes were also on high level.Therefore,under in vitro conditions,the copper content recommended in yak's diet is between 10.0 and 15.0 mg/kg.

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