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Position: Home > Articles > Determination of Content of Flavonoids in Flos Puerariae Journal of Hebei North University(Natural Science Edition) 2007,23 (6) 33-34+38

葛花中总黄酮含量测定

作  者:
边永玲;甄攀;李焕芬;郭春燕;张利民;安方
单  位:
河北北方学院教务处;河北北方学院药学系
关键词:
葛花;芦丁;总黄酮;分光光度法;含量测定
摘  要:
目的:建立用可见分光光度法测定葛花中总黄酮含量的方法.方法:乙醚脱脂,乙醇提取总黄酮.采用NaNO2,Al(NO3)3,NaOH显色,可见分光光度法测定总黄酮.结果:以芦丁为对照品作校正曲线,C=71.103A-0.810,r=0.9998,在12.32~61.60μg/ml范围内线性关系良好.葛花中总黄酮含量为0.75%.结论:方法简便、快速、灵敏、准确,可作为葛花中总黄酮的含量测定方法.
译  名:
Determination of Content of Flavonoids in Flos Puerariae
作  者:
BIAN Yong-ling1,ZHEN Pan2,LI Huan-fen2,GUO Chun-yan2,ZHANG Li-min2,AN Fang1(1.Dean's Office,Hebei North University,Zhangjiakou 075000,Hebei,China;2.Department of Pharmacy,Hebei North University,Zhangjiakou 075000,Hebei,China)
关键词:
Flos Pueraiae;Rutin;flavonoids;spectrophtometry;assay
摘  要:
Objective:To establish a method for determination the content of flavonoids by visible spectrophotometry in the Chinese herbal medicine of Flos Pueraiae.Methods: Flavonoids in Flos Puerariae was extracted with the solvent ethanol after Ether degreasing.The NaNO2-Al(NO3)3-NaOH spectrophotometry was used to determine flavonoids.Results:Rutin was taken as the standard to make the standard curve:C=71.103A-0.81,r=0.9998,the linear range was 12.32~61.60μg·ml-1.The contents of flavoniods in Flos Pueraiae was 0.75%.Conclusion:This method was simple,accurate and suitable for the quality control of the flavonoids in Flos Pueraiae.

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