Position: Home > Articles > Extraction of Genomic DNA from Tea Doping with Maize and Establishment of PCR Detection
Hubei Agricultural Sciences
2013,52
(22)
233-235
掺杂玉米茶叶的基因组DNA提取与PCR检测方法建立
作 者：
余澍琼;张吉红;赵旭东;陈建国
单 位：
临沂市产品质量监督检验所;宁波出入境检验检疫局
关键词：
茶叶(Camellia sinensis);玉米(Zea mays);普通PCR;实时荧光PCR;检测方法
摘 要：
根据玉米内源ZEIN基因建立了茶叶中掺杂玉米的普通PCR和实时荧光PCR检测方法。结果表明,标准CTAB法适合提取茶叶样品基因组DNA,实时荧光PCR的灵敏度能达到0.1%,普通PCR能达到1%,实时荧光PCR是普通PCR的10倍。两种方法都能达到检测要求。
译 名：
Extraction of Genomic DNA from Tea Doping with Maize and Establishment of PCR Detection
作 者：
YU Shu-qiong;ZHANG Ji-hong;ZHAO Xu-dong;CHEN Jian-guo;Ningbo Entry-Exit Inspection and Quarantine Burean;Linyi Institute of Products Oualty Supervision and Inspection;
关键词：
tea(Camellia sinensis);;maize(Zea mays);;ordinary PCR;;real-time PCR;;detection methods
摘 要：
According to the endogenous gene ZEIN of maize, two PCR methods were established. The results showed that standard CTAB method was suitable for genomic DNA extraction of maize. Ordinary PCR had 1% detection sensitivity. The detection sensitivity of real-time PCR was 10 times more than that of ordinary PCR. Two methods can meet requirements for detecting maize in tea in future.
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