作 者：
黄兴;柴志欣;王会;姬秋梅;信金伟;钟金城
单 位：
西藏自治区农牧科学院省部共建青稞和牦牛种质资源与遗传改良国家重点实验室;西南民族大学/青藏高原研究院
关键词：
西藏牦牛;MyoD1基因;PCR-RFLP;生长性状;关联分析
摘 要：
【目的】本研究旨在对西藏牦牛MyoD1基因多态性与体高、体重、体斜长、胸围和管围等生长性状进行关联性分析,以期获得对牦牛重要经济性状具有显著效应的遗传标记。【方法】本研究利用DNA池直接测序、PCR-RFLP与生物信息学技术,对西藏帕里(PL)、斯布(SB)、申扎(SZ)和类乌齐(LWQ)4个牦牛类群(品种)共148头个体的MyoD1基因内含子2及外显子3部分序列进行遗传多态性分析,统计基因和基因型频率,进行Hardy-Weinberg平衡性检测,计算纯合度、杂合度、多态信息含量和有效等位基因数等遗传多态指标,分析不同基因型与体高、体重、体斜长、胸围和管围等生长性状的关联性。【结果】①西藏牦牛MyoD1基因外显子3上存在(C1710T)XmaI酶切位点,多态性检测均存在CC、CT和TT 3种基因型,T/C频率分别为:0.308/0.692、0.400/0.600、0.605/0.395和0.527/0.473,处于中度多态。②适合性检验表明,西藏4个牦牛类群MyoD1基因多态位点均处于Hardy-Weinberg平衡状态。③利用最小二乘拟合线性模型,对各标记基因型生长发育指标(体高、体斜长、胸围、管围和体重)进行差异显著性检验,申扎牦牛该位点TT和CT基因型在生长性状上差异显著(P<0.05),CT基因型胸围均高于CC和TT基因型。【结论】可以将MyoD1上的C1710T基因座作为牦牛经济性状选育的一个重要遗传标记,用于育种改良。
译 名：
Association Analysis between Polymorphism of MyoD1 Gene and Growth Traits in Tibetan Yak
作 者：
HUANG Xing;CHAI Zhi-xin;WANG Hui;JI Qiu-mei;XIN Jin-wei;ZHONG Jin-cheng;Institute of Tibetan Plateau Research, Southwest Minzu University;State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement, Tibet Academy of Agricultural and Animal Husbandry Sciences;
单 位：
HUANG Xing%CHAI Zhi-xin%WANG Hui%JI Qiu-mei%XIN Jin-wei%ZHONG Jin-cheng%Institute of Tibetan Plateau Research, Southwest Minzu University%State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement, Tibet Academy of Agricultural and Animal Husbandry Sciences
关键词：
Tibetan Yak;;MyoD1;;PCR-RFLP;;Growth traits;;Association analysis
摘 要：
【Objective】This experiment was conducted to study the association between the polymorphism of MyoD1 and growth traits, such as body height, weight, body length, chest girth and cannon circumference, in Tibetan yak in order to obtain the genetic markers with significant effect on the important economic traits. 【Method】The yaks population in this study comprised 148 yaks from four varieties(Pali Yak, Sibu Yak, Shenzha Yak and Leiwuqi Yak), then the association analysis between the genetic variation of intron 2 and exon 3 partial sequence of MyoD1 gene and growth traits was carried out using DNA pool sequencing, PCR-RFLP and bioinformatics techniques. In addition, the Hardy-Weinberg balance and the population genetic indexes including gene homozygosity(Ho), gene heterozygosity(He), polymorphism information content(PIC) and effective allelenumber(Ne), were tested or directly calculated. 【Result】The results showed as follows:(i)One polymorphisms were detectedin exon 3 of MyoD1 gene in Tibetan Yak, which exists C and T mutation(C1710 T, named XmaI Locus), three different genotypes with CC, CT and TT were detected from this locus. The T/C allele frequencies in the four varieties Yak were 0.308/0.692, 0.400/0.600, 0.605/0.395, and 0.527/0.473, respectively. Moreover, XmaI locus indicated middle polymorphism(0.25
0.05).(iii)A significant association between the TT and CT genotypes and growth traits were found in Shenzha Yak(P<0.05) using the SPSS(18.0) software and adopting the least square linear fitting method. The individuals with CT genotype had higher chest girth than those with TT and CC genotype. 【Conclusion】The C1710 T locus on MyoD1 can be preliminarily considered as a candidate genetic marker for economic traits breeding in Yak.
