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Position: Home > Articles > Polymorphism of PRKAG3 Gene and its Associations with Meat Quality Traits in Plymouth Rock Heilongjiang Journal of Animal Reproduction 2012 (6) 5-9

芦花鸡PRKAG3基因单核苷酸多态性及与肉质性状的相关性分析

作  者:
哈桑·阿巴克;张永宏;郭将;李传民;马倩;赵衍铜;邱峥艳;张嘉保;赵志辉
单  位:
吉林大学畜牧兽医学院;吉林大学实验动物中心
关键词:
芦花鸡;PRKAG3基因;PCR-SSCP;肉质性状
摘  要:
为研究芦花鸡PRKAG3基因的多态性及与肉质性状相关性,采用PCR-SSCP方法检测PRKAG3基因外显子11的遗传多态性。结果检测到TT、TG和GG三种基因型,测序结果证实在2832位点存在T→G突变,为有意突变,统计分析表明,此多态位点与芦花鸡呈显著相关,pH值为GG基因型极显著低于TG基因型和TT基因型(P<0.01),而TG基因型显著低于TT基因型(P<0.05);其它肉质性状在不同基因型间差异不显著。此结果提示,PRKAG3基因对芦花鸡pH值具有较大的遗传效应,可以初步推断PRKAG3是控制这一性状的众多基因之一,可能是影响芦花鸡pH值的一个主效基因或与主效基因相连锁,可作为选育芦花鸡pH值的分子标记,用于标记辅助选择意义重大。
译  名:
Polymorphism of PRKAG3 Gene and its Associations with Meat Quality Traits in Plymouth Rock
作  者:
HASSAN OSMAN ABBAKER1,ZHANG Yong-hong1,GUO Jiang1,LI Chuan-min1, MA Qian1,ZHAO Yan-tong1,QIU Zheng-yan1,ZHANG Jia-bao2,ZHAO Zhi-hui1*(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jinlin,130062,China; 2.Laboratory Animal Center,Jilin University,Changchun,Jilin,130062,China)
关键词:
Plymouth rock;PRKAG3;PCR-SSCP;Meat Quality Traits
摘  要:
This study was designed to investigate the single nucleotide polymorphism(SNP) by PCR-SSCP of PRKAG3 gene in 87 plymouth rock,at the same time the correlation between the polymorphism and meat quality traits.The result of PCR-SSCP showed that there were three genotypes(TT,TG and GG).The correlation analysis revealed that polymorphism had a extremely significant effect on PH,the plymouth rocks with GG genotype had lower PH than the GT and TT genotype(P<0.01),at the same time,the GT genotype lower than the TT genotype(P<0.05).other meat quality traits of plymouth rock were not significant influence on different genotypes(P>0.05).It could be preliminarily concluded that PRKAG3 gene was probably a major gene or a QTL linked gene which associated with PH and can be chosen as the molecular markers of meat quality in plymouth rock.

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