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Position: Home > Articles > Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource Acta Horticulturae Sinica 2012,39 (5) 985-991

番茄抗叶霉病基因Cf12的分子标记筛选及种质资源鉴定

作  者:
赵婷婷;宋宁宁;姜景彬;张贺;康立功;李景富;许向阳
单  位:
东北农业大学园艺学院
关键词:
番茄;叶霉病;Cf12基因;AFLP分子标记;SCAR
摘  要:
以番茄抗叶霉病的品种05HN36为母本,以感病品种051355为父本配置杂交组合,以亲本及其F2分离群体为研究材料,采用AFLP技术筛选与抗叶霉病基因Cf12连锁的分子标记。通过对545对引物进行筛选,获得了6个连锁的AFLP标记:E86M51-C、E46M50、E95M59-D、E35M81、E78M36-C和E47M50-C,连锁遗传距离分别为5.1、8.9、9.1、9.2、10.2和10.8cM。将E86M51-C转化为SCAR标记并应用于种质资源筛选,获得了16份含有该标记的番茄材料,为抗叶霉病育种提供材料基础。
译  名:
Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource
作  者:
ZHAO Ting-ting,SONG Ning-ning,JIANG Jing-bin,ZHANG He,KANG Li-gong,LI Jing-fu,and XU Xiang-yang (College of horticulture,Northeast Agricultural University,Harbin 150030,China)
关键词:
tomato;Cladosporium fulvum;resistance gene Cf12;AFLP;SCAR;
摘  要:
The study identified the molecular marker linked to Cladosporium fulvum resistant gene Cf12 in tomato. The linked marker was screened with a F2 population between a resistant and a susceptible parent(05HN36 × 051355)by AFLP technology. Six AFLP markers had been identified to linked with Cf12 gene:E86M51-C,E46M50,E95M59-D,E35M81,E78M36-C and E47M50-C. The genetic distances were 5.1,8.9,9.1,9.2,10.2 and 10.8 cM respectively. The AFLP marker E86M51-C was converted into SCAR marker. We obtained 16 cultivars which contained this marker. These cultivars could be used in C. fulvum resistant breeding practice.

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