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当前位置: 首页 > 文章 > Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows 畜牧与生物技术杂志(英文版) 2014,5 (2) 145-153
Position: Home > Articles > Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows Journal of Animal Science and Biotechnology 2014,5 (2) 145-153

Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows

作  者：
Sinéad M. Waters;Kate Keogh;F. Buckley;David A. Kenn
单  位：
Teagasc Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Dunsany, Ireland;Teagasc Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Dunsany, Irelan;Teagasc Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Fermoy, Ireland
关键词：
Bovine;Duodenum;Gene expression;Nutrient transporter
摘  要：
Background: Studies have shown clear differences between dairy breeds in their feed intake and production efficiencies. The duodenum is critical in the coordination of digestion and absorption of nutrients. This study examined gene transcript abundance of important classes of nutrient transporters in the duodenum of non lactating dairy cows of different feed efficiency potential, namely Holstein-Friesian (HF), Jersey (JE) and their F, hybrid. Duodenal epithelial tissue was collected at slaughter andstored at -80°C Total RNA was extracted from tissue and reverse transcribed to generate cDNA. Gene expression of the following transporters, namely nucleoside; amino acid; sugar; mineral; and lipid transporters was measured using guantitative real-timeRT-PCR. Data were statistically analysed using mixed models ANOVA in SAS. Orthogonal contrasts were used to test for potential heterotic effects and spearman correlation coefficients calculated to determine potential associations amongst gene expressionvalues and production efficiency variables.Results: While there were no direct effects of genotype on expression values for any of the genes examined, there was evidence for a heterotic effect (P<0.05) on ABCG8, in the form of increased expression in theF, genotype compared to either of the two parent breeds. Additionally, a tendency for increased expression of the amino acid transporters, SLC3A1 (P = 0.072), SLC3A2 (P = 0.081) and 5LC6A14 (P= 0.072) was also evident in the F, genotype. A negative (P<0.05) association was identified between the expression of the glucose transporter gene SLC5A1 and total lactational milk solids yield, corrected for body weight. Positive correlations (P< 0.05) were also observed between the expression values of genes involved in common transporter roles.Conclusion: This study suggests that differences in the expression of sterol and amino acid transporters in the duodenum could contribute towards the documented differences in feed efficiency between HF, JE and their F, hybrid. Furthermore, positive associations between the expression of genes involved in common transporter roles suggest that these may be co-regulated. The study identifies potential candidates for investigation of genetic variants regulating nutrient transport and absorption in the duodenum in dairy cows, which may be incorporated into future breeding programmes.

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