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Functional Marker Development and Effect Analysis of Grain Size Gene GW2 in Extreme Grain Size Germplasm in Rice

作者：

Yadong Zhang;Jufeng Zheng;Yanli Li;Chao Zhao;Tao Chen;Zhao QingYong;Zhen Zhu;Lihui Zhou;Shixiang Yao;Liyan Zhao;Yu Xing;Cailin Wan

单位：

Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R & D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, Chin;Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R & D Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China

关键词：

rice;grain size;GW2 gene;derived cleaved amplified polymorphic sequence;phenotyp

摘要：

GW2 is an important gene that regulates grain width and weight. We used cDNA clone to obtain the sequences of GW2 from large-and small-grained rice varieties, TD70 and Kasalath, respectively. Then, we developed a dCAPS (derived cleaved amplified polymorphic sequence) marker on the basis of the sequence difference between functional and nonfunctional GW2 genes to analyze the genotypes and phenotypes of recombinant inbred lines. Results showed that the sequence of GW2(TD70) had a single nucleotide deletion at site 316 that generates a termination codon. This codon terminated the GW2 protein in advance. By contrast, the sequence of GW2(Kasalath) encoded an intact protein. A novel dCAPS marker was designed in accordance with a base A deletion at site 316 of the sequence. After the PCR product was digested by ApoI, TD70 showed 21 and 30 bp fragments, and Kasalath showed a 51 bp fragment. Up to 82 lines contained GW2(TD70), and 158 lines contained GW2(Kasalath). The lines that contained TD70 alleles displayed substantial increases in width and 1000-grain weight. This result suggested that GW2 played a critical role in rice breeding.

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Functional Marker Development and Effect Analysis of Grain Size Gene GW2 in Extreme Grain Size Germplasm in Rice

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