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An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China

作者：

Xiaofang Li;Hongbin Yin;Jian‐Qiang S

单位：

Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003 (China;Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China);Centre for Mined Land Rehabilitation, Sustainable Minerals Institute, the University of Queensland, St Lucia Qld 4072 (Australia); Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003 (China)

关键词：

cu;resistant;microorganisms;primers;soil;quantif

摘要：

Understanding the mechanisms of Cu pollution-induced community tolerance (PICT) in soil requires the characterization of Cu-resistant microorganisms at a community level using modern molecular tools. A primer pair (copAF2010 (5'-TGCAC CTGAC VGGSC AYAT-3')/copAR2333 (5'-GVACT TCRCG GAACA TRCC-3')) tentatively targeting Pseudomonas-like Cu-resistant microorganisms was designed in this study. The specification of the primers was tested through conventional polymerase chain reaction (PCR) and the construction of a Pseudomonas-like copA gene fragment library, and then the primers were used to quantify the Cu-resistant microorganisms using quantitative PCR technique. A significant increase of Cu-resistant microorganisms targeted by the primers was observed in a paddy soil from Jiaxing, China which has been exposed to one-year Cu contamination. The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution.

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An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China

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