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当前位置: 首页 > 文章 > Genetic diversity analysis of Lepidium sativum (Chandrasur) using inter simple sequence repeat (ISSR) markers 林业研究(英文版) 2015,26 (1)
Position: Home > Articles > Genetic diversity analysis of Lepidium sativum (Chandrasur) using inter simple sequence repeat (ISSR) markers Journal of Forestry Research 2015,26 (1)

Genetic diversity analysis of Lepidium sativum (Chandrasur) using inter simple sequence repeat (ISSR) markers

作  者：
Amandeep Kaur;Rakesh Kumar;Shikha Rani;Anita Grewa
单  位：
Department of Biotechnology, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra, India;Department of Biotechnology, University Institute of Engineering & Technology, Kurukshetra University, Kurukshetra, Indi
关键词：
ISSR;Genetic diversity;Polymorphism;Lepidium sativum;Cluster analysis;MOLECULAR MARKERS;RAPD MARKER
摘  要：
Lepidium sativum (commonly known as garden cress) belongs to the family Brassicaceae. It is a fast-growing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic, hypoglycemic, nephrocurative and nephroprotective activities. In the present study, we assessed the genetic diversity of various genotypes of L. sativum using inter-simple sequence repeat (ISSR) markers. Out of 41 ISSR primers screened, 32 primers showed significant, clear and reproducible bands. A total of 510 amplified bands were obtained using 32 ISSR primers, out of which 422 bands were polymorphic and 88 bands were monomorphic. The percentage of polymorphism was found to be 82. A total of 35 unique alleles ranging insize from 200 to 2,900 bp were observed. Cluster analysis based on unweighted pair-group method, arithmetic mean divided the 18 genotypes into two main clusters, with the first having only HCS-08 genotype of L. sativum and other having all of the other 17 genotypes. The Jaccard similarity coefficient revealed a broad range 32-72 % genetic relatedness among the 18 genotypes.

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