作 者：
Marcelo Luiz de Laia;Acelino C. Alfenas;Sérgio Hermínio Brommonschenkel;Shinitiro Oda;Eduardo José de Melo;Inaê Mariê de Araújo Silva‐Cardoso;Janaína Fernandes Gonçalves;Ariadne Marque
单 位：
Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, Brazil;Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brazil;Suzano Bahia Sul Celulose S/A, Suzano, Brazil;Departamento de Engenharia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brazi
关键词：
Cloning;Conserved domains;RGAs;Rust;Eucalyptus;NUCLEOTIDE-BINDING SITE;ARABIDOPSIS-THALIANA;PATHOGEN RESISTANCE;POPULUS-TREMULA;PINUS-MONTICOLA;DISEASE;LOCI;IDENTIFICATION;EXPRESSION;HOMOLOG
摘 要：
Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode proteins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and characterized gene-resistant analogs (RGAs) from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the pGEM-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previously identified gene-resistant diseases. A BLASTX search in GenBank revealed high similarities among the conserved domains of these cloned genes with RGA genes. Some clones, however, showed no significant similarity with DNA sequences in GenBank. Southern blotting analysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1 (Ppr1) in a population study.
