Weijie Yao;Yaru Fu;Zhang Yan-fu;Hui'e L

Agricultural and Animal Husbandry College, Tibet University, Linzhi, Tibet, People’s Republic of Chin;Key Laboratory of Forest Ecology in Tibet Plateau (Tibet University), Ministry of Education, Linzhi, Tibet, People’s Republic of China

Proline content;Expression profile;Drought stress;Salt stress;Cold stress;Heat stress;BINDING TRANSCRIPTION FACTORS;SALT TOLERANCE;FUNCTIONAL-CHARACTERIZATION;DROUGHT;DEHYDRATION;ARABIDOPSIS;OVEREXPRESSION;RESPONSES;PROLIN

Sophora moorcroftiana is an endemic, drought-resistant shrub that grows in Tibet and has some degree of resistance to salt, cold, heat, and drought. In the present study, four dehydration responsive element-binding (DREB) genes (SmDREB1, SmDREB2, SmDREB and SmDREB1) were isolated from S. moorcroftiana for the first time and their expression and proline content under abiotic stress were analyzed. Proline accumulated in seedlings under drought, salt, cold, and heat stress treatments. The four genes were variously expressed in response to the four abiotic stresses. SmDREB1 was induced by drought, cold, and heat stresses; SmDREB2 and SmDREB4 were both induced by salt, cold, and heat stresses, whereas SmDREB3 was induced by drought and heat stresses. Thus, these four genes may participate in conferring tolerance to these four abiotic stresses and are candidate genes for genetic engineering in the future.