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Position: Home > Articles > In vitro germination and shoot proliferation of the threatened species Cinchona officinalis L (Rubiaceae). Journal of Forestry Research 2016,27 (6)

In vitro germination and shoot proliferation of the threatened species Cinchona officinalis L (Rubiaceae).

作者：

Rosa Armijos-González;César Pérez-Ruí

单位：

Departamento de Biotecnología y Biología Vegetal, E.T.S. Ingenieros Agrónomos, Universidad Politécnica de Madrid, Madrid, Spai;Departamento de Ciencias Naturales, Universidad Técnica Particular de Loja, Loja, Ecuador

关键词：

Callus;Cinchona officinalis;Germination;Phenols;Rubiaceae;Shoot;CELL-SUSPENSION CULTURES;STRICTOSIDINE SYNTHASE;LIQUID-CHROMATOGRAPHY;QUINOLINE ALKALOIDS;GROWTH;REGENERATION;PRECURSORS;SHAK

摘要：

Cinchona officinalis (Rubiaceae) is an endemic species of the Loja Valley in southern Ecuador with medicinal uses. Because of over-exploitation in the nineteenth century and more recent disturbances to its ecosystem, C. officinalis populations are threatened. Currently, natural regeneration of the populations is low, despite its high plant regeneration and seed formation capacity. In the present study, an efficient protocol for germination, shoot proliferation and plantlets regeneration was developed for this species. Phenolic content and germination rate of C. officinalis seeds were compared with a control species, C. pubescens. Nodal segments from seedlings of C. officinalis were cultured on Gamborg medium supplemented with different combinations of plant growth regulators. Because the phenol content is high in C. officinalis, the phenolic should be removed with hydrogen peroxide or water washes to stimulate germination. Shoots and callus developed from nodal segments within 45 days using most of the tested combinations of plant growth regulators. The best rates of shoot proliferation, callus formation and adventitious buds were obtained in medium supplemented with 5.0 mg L-1 6-benzyl-aminopurine and 3.0 mg L-1 indole-3-butyric acid.

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In vitro germination and shoot proliferation of the threatened species Cinchona officinalis L (Rubiaceae).

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