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Position: Home > Articles > In vitro propagation of conifers using mature shoots Journal of Forestry Research 2018,29 (3)

In vitro propagation of conifers using mature shoots

作者：

Mostafa Khoshhal Sarmas

单位：

Department of Horticultural Science, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), Gorgan, Ira

关键词：

Conifers;Decontamination;Micropropagation;Tissue culture;PINUS-PINEA COTYLEDONS;AGROBACTERIUM-MEDIATED TRANSFORMATION;ADVENTITIOUS BUD FORMATION;BETA-LACTAM ANTIBIOTICS;TOBACCO TISSUE-CULTURES;SOMATIC EMBRYOGENESIS;PLANT-REGENERATION;INVITRO-PROPAGATION;ZYGOTIC EMBRYOS;CALLUS-CULTURE

摘要：

Micropropagation mostly leads to the production of innumerable true-to-type plants. However, establishing pathogen-free explants through in vitro culture requires a precise management of time for the exposure of explants to antimicrobial chemicals. The application of antimicrobial chemicals must also be managed to impose the least injury on explants. This review discusses the contributions of micropropagation procedures, explant types, subculture duration, media ingredients and plant growth regulators to the in vitro response of conifer explants. Even though regeneration from mature conifer explants such as mature shoots are laborious, the chances of variation, induced in vitro, are unlikely.

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In vitro propagation of conifers using mature shoots

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