Setareh Mirzavand;Karim Sorkheh;Mohammad Reza Siahpoos

Shahid Chamran University of Ahvaz.;Shahid Chamran University of Ahvaz

Genetic relationship;Classification;Genetic resources;PAL;Phenylalanine ammonia-lyase gene;GENOME-WIDE CHARACTERIZATION;PEROXIDASE GENE;DIFFERENTIAL EXPRESSION;ARABIDOPSIS-THALIANA;FAMILY;POLYMORPHISM;GERMPLASM;INFERENCE;TOOLBOX;PAL

Information on population genetic structure and crop genetic diversity is important for genetically improving crop species and conserving threatened species. The PAL gene sequence is part of a multigene family that can be utilized to design DNA marker systems for genetic diversity and population structure investigation. In the current study, genetic diversity and population structure of 100 accessions of wild Pistacia species were investigated with 78 PAL markers. A protocol for using PAL sequences as DNA markers was developed. A total of 313 PAL loci were recognized, showing 100% polymorphism for PAL markers. The PAL markers produced relatively more observed and effective alleles in Pistacia falcata and Pistacia atlantica, with a higher Shannon's information index and expected heterozygosity in P. atlantica, Pistacia vera and Pistacia mutica. Pairwise assessment of Nei's genetic distance and genetic identity between populations revealed a close association between geographically isolated populations of Pistacia khinjuk and Pistacia chinensis. The accessions of wild Pistacia species had more genetic relationship among studied groups of species. Analysis of molecular variance indicated 19% among-population variation and 81% within-population variation for the PAL gene based DNA marker. Population structure analysis based on PAL revealed four groups with high genetic admixture among populations. The results establish PAL markers as a functional DNA marker system and provide important genetic information about accessions from wild populations of Pistacia species.