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当前位置: 首页 > 文章 > Effect of the thin cell layer technique in the induction of somatic embryos in Pinus patula Schl. et Cham 林业研究(英文版) 2019,30 (4)
Position: Home > Articles > Effect of the thin cell layer technique in the induction of somatic embryos in Pinus patula Schl. et Cham Journal of Forestry Research 2019,30 (4)

Effect of the thin cell layer technique in the induction of somatic embryos in Pinus patula Schl. et Cham

作  者：
Marco A. Ramírez-Mosqueda;Lourdes G. Iglesias‐Andreu;Arturo Alonso Armas-Silva;Esmeralda J. Cruz-Gutiérrez;José Fernando De La Torre-Sánchez;Otto Raúl Leyva‐Ovalle;Carlos M. Galán-Páe
单  位：
Facultad de Ciencias Biológicas y Agropecuarias Región Córdoba-Orizaba, Universidad Veracruzana, Amatlan de los Reyes, Mexico;Centro Nacional de Recursos Genéticos-INIFAP, Tepatitlán de Morelos, Mexico;Facultad de Ciencias Biológicas y Agropecuarias Región Córdoba-Orizaba, Universidad Veracruzana, Amatlan de los Reyes, Mexic;Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Xalapa, Mexico
关键词：
Pinus patula;Somatic embryos;Micropropagation;Thin cell layer;Maturation;PLANT-REGENERATION;EMBRYOGENESIS;PROPAGATION;CULTURE;SEE
摘  要：
Pinus patula is a species commonly used for reforestation in Mexico. However, efficient methods for the mass production seedlings are required. Micropropagation particularly by somatic embryogenesis provides an option for the rapid multiplication of high-quality, genetically improved material. This study induces somatic embryogenesis in this species using the thin cell layer (TCL) technique. Two sources of explants (complete immature embryos; lTCL segments from immature embryos) were evaluated. The efficiency of TCL from longitudinal sections [lTCL] and transverse [tTCL] was evaluated. The results show using thin cell layers from immature embryos cultivated in 16 light/8 dark hours achieves induction of somatic embryos. A higher percentage of embryogenic callus was obtained when tTCL segments were used as an explant source. These results produced somatic embryos from tTCL segments of an immature embryo without germinating the seed, making the process more time efficient. In addition, this technique can be used to generate somatic embryogenesis in forest species that have low germination rates.

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