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当前位置: 首页 > 文章 > Overexpression of PtPEPCK1 gene promotes nitrogen metabolism in poplar 林业研究(英文版) 2019,30 (6)
Position: Home > Articles > Overexpression of PtPEPCK1 gene promotes nitrogen metabolism in poplar Journal of Forestry Research 2019,30 (6)

Overexpression of PtPEPCK1 gene promotes nitrogen metabolism in poplar

作  者：
Lina Wang;Mingyue He;Song Chen;Kean Wang;Donghai Cui;Xin Huang;Lijie Li
单  位：
College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar, People’s Republic of China;State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, People’s Republic of China;College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar, People’s Republic of Chin
关键词：
Amino acid metabolism;Metabolome;Nitrogen metabolism;Phosphoenolpyruvate carboxylase kinase;Transcriptome;Urea cycle;PHOSPHOENOLPYRUVATE CARBOXYKINASE;AMINO-ACID;GLUCONEOGENESIS;RESPONSES;LEAVES;CYCLE;PHOTOSYNTHESIS;TRANSFORMATION;ASPARTATE;GLUTAMIN
摘  要：
To understand the function of phosphoenolpyruvate carboxylase kinase, we introduced PtPEPCK1 gene under the control of 35S promoter into 84K poplar (Populus albaxP. glandulosa). PtPEPCK1 gene is well-known for its role in gluconeogenesis. However, our data confirmed that it has significant effects on amino acid biosynthesis and nitrogen metabolism. Immunohistochemistry and fluorescence microscopy indicate that PtPEPCK1 is specifically expressed in the cytoplasm of the spongy and palisade tissues. Overexpression of PtPEPCK1 was characterized through transcriptomics and metabolomics. The metabolites concentration of the ornithine cycle and its precursors also increased, of which N-acetylornithine was up-regulated almost 50-fold and ornithine 33.7-fold. These were accompanied by a massive increase in levels of several amino acids. Therefore, overexpression of PtPEPCK1 increases amino acid levels with urea cycle disorder.

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