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当前位置: 首页 > 文章 > Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility 畜牧与生物技术杂志(英文版) 2019,10 (1)
Position: Home > Articles > Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility Journal of Animal Science and Biotechnology 2019,10 (1)

Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility

作  者：
Maria-Portia B. Nagata;Junki Egashira;Naoto Katafuchi;Kenji Endo;Kenichi Ogata;Kenichi Yamanaka;T. Yamanouchi;Hideo Matsuda;Y. Hashiyada;Kenichi Yamashit
单  位：
Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, 840-8502, Japan;Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku-machi, Tosu, Saga, 841-0052, Japan;Saga Prefectural Livestock Experiment Station, 23242-2 Yamauchi-machi, Miyano, Takeo, Saga, 849-2305, Japan;Morinaga Dairy Service Co. Ltd., 1-159 Toyoharaotsu, Nasu-gun Nasu-machi, Tochigi, 329-3224, Japan;National Livestock Breeding Center (NLBC), 1 Odakurahara, Odakura, Nishigo-mura, Nishishirakawa-gun, Fukushima, 961-8511, Japan;Advanced Manufacturing Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku-machi, Tosu, Saga, 841-0052, Japa
关键词：
Cryopreservation;Fertility;Livestock;Rheotaxis;Spermatozoa;Thermotaxi
摘  要：
BackgroundThe application of cryopreservation and artificial insemination technology have contributed to the advancement of animal reproduction. However, a substantial proportion of spermatozoa undergoes alterations and loses their fertility during cryopreservation, rendering the frozen-thawed semen impractical for routine use. Cryopreservation is known to reduce sperm lifespan and fertility. Variation in cryosurvival of spermatozoa from different sires and even with the individual sire is common in artificial insemination (AI) centers. Our goal is to improve post-thawed semen quality by optimization of cryopreservation technique through sperm selection prior to cryopreservation process.ResultsOur strategy of sperm selection based on rheotaxis and thermotaxis (SSRT) on macrosale in a rotating fluid flow demonstrated the ability to maintain the original pre-freezing structural integrity, viability and biological function related to fertilization competence. This strategy has a positive effect on the cryosurvival and fertilizing abilities of spermatozoa as supported by the improvement on pregnancy rate of Japanese Black heifers and Holstein repeat breeders. This technique protected further sublethal damage to bovine spermatozoa (higher % cryosurvival than the control) and resulted in the improvement of DNA integrity. Prefreeze selected spermatozoa demonstrated slower and controlled capacitation than unprocessed control which is thought to be related to sperm longevity and consequently to appropriate timing during in vivo fertilization.ConclusionsThese results provide solid evidence that improvement of post-thawed semen quality by SSRT method is beneficial in terms of cryosurvival, longevity of post-thawed sperm, and optimization of in vivo fertilization, embryo development and calving as supported by the favorable results of field fertility study.

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