中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > Decreased morphogenetic potential in peach palm stem-like cells in long-term in vitro conditions 林业研究(英文版) 2020,31 (2)
Position: Home > Articles > Decreased morphogenetic potential in peach palm stem-like cells in long-term in vitro conditions Journal of Forestry Research 2020,31 (2)

Decreased morphogenetic potential in peach palm stem-like cells in long-term in vitro conditions

作  者：
Edgard Graner;Gilvano Ebling Brondani;Cristina Vieira de Almeida;Katherine Derlene Batagin-Piotto;Marcílio de Almeid
单  位：
“Luiz de Queiroz” College of Agriculture, University of São Paulo, Piracicaba, Brazil;“Luiz de Queiroz” College of Agriculture, University of São Paulo, Piracicaba, Brazi;Department of Forest Sciences, Câmpus Universitário, Federal University of Lavras, Lavras, Brazil;In Vitro Palm (Consulting, Study and Biological Development Ltda.), Piracicaba, Brazil
关键词：
Micropropagation;Multipotency;Pluripotency;Totipotency;Pre-procambial cells;Peach palm;Bactris gasipaes;SOMATIC EMBRYOGENESIS;PLANT;REGENERATION;PLURIPOTENT;ORGANOGENESIS;CULTUR
摘  要：
Peach palm (Bactris gasipaes Kunth) has been micropropagated from pre-procambial cells that provide stem-like cell niches, (i.e., pre-procambial cells), multipotent, pluripotent and totipotent for direct vascularization, adventitious buds and somatic embryogenesis, respectively. The direct induction of adventitious buds and somatic embryogenesis reduces the frequency of mutations when compared to indirect morphogenesis. Long-term in vitro cultivation of perennial species such as peach palm cause the clones to age and deteriorate; however, the consequences for morphogenesis potential are not fully clear. The morphogenic potential of peach palm clones established and in vitro cultivated for 8 years (regeneration of adventitious buds without callus formation) was investigated in leaves, roots and stem bases using histological and histochemical analyses. Data from long-term cultures (8-years-old) was compared to data from short-term cultures (1-year-old). Morphogenic pathways monitoring for direct induction of somatic embryos and adventitious buds revealed a strong morphogenic reduction potential in the pre-procambial cells, parenchyma cells in the proximal region of stem bases, and external cells of leaf sheaths. Initial cells of shoot apical meristems and pre-procambial cells commit cell reprogramming to the undifferentiated state and subsequent acquisition of cellular competence. These results are applicable in the micropropagation of peach palm, with consideration to obtaining clones and their long-term in vitro culture.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46