中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > Short-term transcriptomic responses of Populus euphratica roots and leaves to drought stress 林业研究(英文版)
Position: Home > Articles > Short-term transcriptomic responses of Populus euphratica roots and leaves to drought stress Journal of Forestry Research

Short-term transcriptomic responses of Populus euphratica roots and leaves to drought stress

作  者：
Peipei Jiao;Zhihua Wu;Xu Wang;Zhenbo Jiang;Yanqin Wang;Hong Liu;Rui Qin;Zhijun L
单  位：
Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China & Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Sciences, South-Central University for Nationalities, Wuhan, People’s Republic of China; Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin, Xinjiang Production and Construction Group, Alar, People’s Republic of Chin; Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin, Xinjiang Production and Construction Group, Alar, People’s Republic of China;College of Life Sciences, Tarim University, Alar, People’s Republic of China
关键词：
Drought stress;RNA-seq;Populus euphratica;qRT-PCR;WHEAT LEAVES;GENOME;PROTEINS;GENES;COLD;SALT;TOO
摘  要：
Populus euphratica Oliv., the Euphrates poplar, is the tallest tree species in the arid desert areas of Northwest China. Investigation of its drought-resistance genes is valuable to increase understanding of drought resistance mechanisms. RNA-seq of leaves and roots under drought simulation by 25% polyethylene glycol-6000 (PEG 6000) were performed at 0, 4, and 12 h. Leaves and roots responded differently to drought via differentially upregulated and downregulated genes; more genes were downregulated than upregulated in both leaves and roots. Additionally, these differentially expressed genes were enriched in different GO terms and KEGG pathways. For example, GO term 'response to organic substance' and KEGG pathway 'nitrogen metabolism' were enriched for drought-stressed leaves, while GO term 'cell wall organization or biogenesis' and KEGG pathway 'zeatin biosynthesis' were enriched for drought-stressed roots. The enrichment of the GO term 'enzyme linked receptor protein signalling pathway' in both leaf and root drought responses suggests that these tissues may also have similar mechanisms. However, roots under drought stress for four hs responded by activating programed cell death. The KEGG pathway 'plant hormone signal transduction' was detected for 4- and 12-h drought-stressed leaves and 12-h drought-stressed roots, suggesting that plant hormone signal transduction plays an important role in both roots and leaves. GO enrichment of upregulated and downregulated genes for leaves and roots reflect differentially regulatory mechanisms of response to drought stress via different biological processes such as the regulation of photosynthesis and auxin signalling pathway in leaves, and the regulation of defence response and water homeostasis in roots. Fifteen candidate genes, including transcription factors, protein kinase, transporter, late embryogenesis abundant protein and mannitol dehydrogenase, were further selected to determine their response to drought using qRT-PCR. The results show that the expression patterns of 13 of the 15 genes correspond to the RNA-seq results. This study provides new insights into the drought response mechanism of P. euphratica and suggests new candidate gene resources.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46