Zhang Dong;Ouyang Shu-hong;Wang Li-li;Cui Yu;Wu Qiu-hong;Liang Yong;Wang Zhen-zhong;Xie Jing-zhong;Zhang De-yun;Wang Yong;Chen Yong-xing;Liu Zhi-yong

China Agr Univ, Dept Plant Genet & Breeding, Key Lab Crop Heterosis Res & Utilizat, State Key Lab Agrobiotechnol,Beijing Key Lab Crop, Beijing 100193, Peoples R China

wild emmer;powdery mildew resistance gene;Pm36;comparative genonnics

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most devastating wheat diseases. Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is a promising source of disease resistance for wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09, originating from wild emmer wheat, has been transferred into the hexaploid wheat line WE4 through crossing and backcrossing. Genetic analyses indicated that the powdery mildew resistance was controlled by a single dominant gene, temporarily designated MIWE4. By mean of comparative genomics and bulked segregant analysis, a genetic linkage map of MIWE4 was constructed, and MIWE4 was mapped on the distal region of chromosome arm 5BL. Comparative genetic linkage maps showed that genes MIWE4, Pm36 and MI3D232 were co-segregated with markers XBD37670 and XBD37680, indicating they are likely the same gene or alleles in the same locus. The co-segregated markers provide a starting point for chromosome landing and map-based cloning of MIWE4, Pm36 and MI3D232.