Guan Ying-li;Yang RongYan;Haifeng Zhan

Siping Institute for the Control of Pharmaceutical and Food, Siping, Jilin 136001, China;Siping Institute for the Control of Pharmaceutical and Food, Siping, Jilin 136001, Chin

clematis hexapetala;oleanolic acid;stem;determination;mm;root

[Objective] To determine the content of oleanolic acid in the roots and stem of Clematis hexapetala, so as to provide theoretical evidence for the further development of Clematis hexapetala resources. [Method] HPLC conditions were as follows: YW-C18 column (4.6 mm×150 mm, 5 μm), mobile phase of methanol-water (V ∶ V=90∶ 10), detection wavelength of 220 nm, sampling amount of 10 μl, flow rate of 1.0 ml/min and column temperature of 25 ℃. [Result] The content of oleanolic acid in roots and stem of Clematis hexapetala were 0.175 2% and 0.096 3% respectively. [Conclusion] Simple and rapid in operations and accurate in determination, the method established in this study is favorable for the determination of oleanolic acid in Clematis hexapetala and provides reference for the assessment of the medicinal value of Clematis hexapetala stem, as well as offers scientific basis for the further development and quality control of Clematis hexapetala resources.