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农业科学学报 (英文)
2013,12
(4)
Position: Home > Articles > Molecular Cloning and Characterization of Three Novel Genes Related to Fatty Acid Degradation and Their Responses to Abiotic Stresses in Gossypium hirsutum L.
Journal of Integrative Agriculture
2013,12
(4)
Molecular Cloning and Characterization of Three Novel Genes Related to Fatty Acid Degradation and Their Responses to Abiotic Stresses in Gossypium hirsutum L.
作 者:
Dong Jia;Wei Li-bin;Hu Yan;Guo Wang-zhen
单 位:
Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Hybrid Cotton R&D Engn Res Ctr, Minist Educ,Coll Agr, Nanjing 210095, Jiangsu, Peoples R China
关键词:
genes related to fatty acid degradation;molecular cloning;expression analysis;abiotic stress;cotton (Gossypium hirsutum L.)
摘 要:
Fatty acid metabolism is responsible not only for oilseed metabolism but also for plant responses to abiotic stresses. In this study, three novel genes related to fatty acid degradation designated GhACX, Gh4CL, and GhMFP, respectively, were isolated from Gossypium hirsutum acc. TM-1. The phylogenetic analysis revealed that amino acid sequences of GhACX and GhMFP have the highest homology with those from Vitis vinifera, and Gh4CL has a closer genetic relationship with that from Camellia sinensis. Tissue- and organ-specific analysis showed that the three genes expressed widely in all the tested tissues, including ovules and fiber at different developing stages, with expressed preferentially in some organs. Among them, GhACX showed the most abundant transcripts in seeds at 25 d post anthesis (DPA), however, GhMFP and Gh4CL have the strongest expression level in ovules on the day of anthesis. Based on real-time quantitative RT-PCR, the three genes were differentially regulated when induced under wounding, methyl jasmonate (MeJA), cold, and abscisic acid (ABA) treatments. The characterization and expression pattern of three novel fatty acid degradation related genes will aid both to understand the roles of fatty acid degradation related genes as precursor in stress stimuli and to elucidate the physiological function in cotton oilseed metabolism.
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Molecular Cloning and Characterization of Three Novel Genes Related to Fatty Acid Degradation and Their Responses to Abiotic Stresses in Gossypium hirsutum L. [Jingao Dong, Libin Wei, Yan Hu, Wangzhen Gu] 农业科学学报(英文版) 2013,12 (4)