Linlin Chen;Yan Zhong;Xiangqin Ouyang;Chunfeng Wang;Lanmei Yin;Jing Huang;Yali Li;Qiye Wang;Junyan Xie;Pengfei Huang;Huansheng Yang;Yulong Yi

Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center for Healthy Breeding of Livestock and Poultry, Changsha, Hunan, 410125, China; National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China;Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China; National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, Chin

Beta-alanine;Immune performance;Intestinal development;Weaned pigle

Beta-alanine is an important amino acid involved in several metabolic reactions in the body. The study aimed to investigate the effect of β-alanine supplementation on intestinal development and the immune performance of weaned piglets. Thirty-two 21-day-old healthy weaned piglets (half female and half male; Duroc × Landrace × Yorkshire) with an initial body weight of 8.11 ± 0.21 kg were randomly divided into 4 groups with 8 replicates of 1 pig each. The control group was fed a basal diet and the three experimental treatment groups were fed diets supplemented with 300, 600 and 1,200 mg/kg β-alanine, respectively. The trial lasted 28 days and the diets fed were divided into 2 phases: the late lactation period (day 1 to 14) and the nursery period (day 15 to 28), during which the weaned piglets had free access to food and water. The regulatory effects of β-alanine were further investigated in vitro using organoids obtained from the jejunum of piglets. In vivo, the addition of β-alanine to the diet had no significant effect on the growth performance of weaned piglets (P > 0.05), but significantly reduced serum levels of immunoglobulin G (IgG) (P < 0.01), immunoglobulin M (IgM) (P = 0.005), and complement 3 (C3) (P = 0.017). The serum interleukin- 6 (IL-6) levels (P < 0.01) were significantly reduced in the 1,200 mg/kg treatment group. The addition of β-alanine increased ileal villus height, with the most significant effect at a concentration of 300 mg/kg (P = 0.041). The addition of 600 mg/kg β-alanine significantly up-regulated the expression of superoxide dismutase (SOD) activity (P = 0.020) and the zonula occludens-1 (ZO-1) gene (P = 0.049) in the jejunum. Diets supplemented with 300 mg/kg β-alanine significantly increased the number of Ki67 positive cells in the jejunal crypts (P < 0.01). In vitro, β-alanine increased the organoid budding rates (P = 0.001) and the budding height of the crypt significantly (P = 0.004). In conclusion, β-alanine can improve intestinal morphology and barrier function, reduce inflammatory responses and alleviate the adverse effects of weaning stress on piglet intestinal health.