Zhao Guan

Hebei Technology and Engineering School,Baoding 071002,Chin

zg;spot blotch;pakchoi;disease;antibacterial activity;screenin

[Objective] The purpose was to screen bacterium with antagonistic effect against pathogen of spot blotch disease in pakchoi in vegetable field.[Method] More than 200 strains of bacteria which could produce spore were isolated from soil in different places.Through screening and rescreening,the bacteria with higher antibacterial activity were conducted observation about thallus shapes and colony characters,a series of physiological and biochemical tests were performed.[Result] Rescreening results indicated that the strains including ZG-10,ZG-19,ZG-59,ZG-72 and ZG-31 had significant antibacterial activity,which had very high research value and good application prospect for biocontrol on spot blotch disease in pakchoi;the strain ZG-10 was identified to be Bacillus subtilis.[Conclusion] The strain ZG-10 had biocontrol potential and good development prospect.This research laid certain basis for subsequent research and strated a new way for the application of antagonistic strain and proteinum polypeptide in agriculture.