Wang FuKe;Yan QianShun;Zheng Zhi-xian

,YAN Qian-shun2,ZHENG Zhi-xiang2

achnatherum inebrians(hance) keng;method;alkaloids;alkaloid;60;swainsonin

[Objective]To study the ultrasonic extraction technology of alkaloid from Achnatherum inebrians(Hance) Keng. [Method]40 kHz ultrasonic aqueous medium was used to treat Achnatherum inebrians(Hance) Keng powder,the filtrate was obtained by removing the impurities by water extraction method,and then extracted respectively with the solvents of chloroform,ethyl acetate and n-butanol. And the alkaloids were obtained in the case of solvent recovery under vacuum distillation. The swainsonine was analyzed by the method of thin-layer chromatography and infrared spectrum. [Result]The optimal combinations of the factors were A1 B2 C2 D2 ,and the optimum extraction conditions were as follows: 1∶ 7. 5 (g /ml) of solid-liquid ratio,60 ℃ of temperature,250 W of ultrasonic power and 60 min of ultrasonic time,under those conditions,the extraction rate was 2. 06 mg /g from dried A. inebrians; and 14. 04 mg /g from fresh A. inebrians. Identification results showed that the mixed alkaloids of Achnatherum inebrians(Hance) Keng contained swainsonine. [Method]This method was rapid and efficient with high accuracy,therefore it could be applied to the extraction of alkaloids from Achnatherum inebrians(Hance) Keng.