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当前位置: 首页 > 文章 > Enhanced resistance to Botrytis cinerea and Rhizoctonia solani in transgenic broccoli with a Trichoderma viride endochitinase gene 农业科学学报 (英文) 2015,14 (3)
Position: Home > Articles > Enhanced resistance to Botrytis cinerea and Rhizoctonia solani in transgenic broccoli with a Trichoderma viride endochitinase gene Journal of Integrative Agriculture 2015,14 (3)

Enhanced resistance to Botrytis cinerea and Rhizoctonia solani in transgenic broccoli with a Trichoderma viride endochitinase gene

作  者：
Yu Ya;Zhang Lei;Lian Wei-ran;Xu Feng-feng;Li Shuang-tao;Xiang Juan;Zhang Guo-zhen;Hu Zan-min;Zhao Bing;Ren Shu-xin;Guo Yang-dong
单  位：
Wuhan Inst Bioengn, Dept Biol Sci & Technol, Wuhan 430415, Peoples R China;Virginia State Univ, Sch Agr, Petersburg, VA 23806 USA;China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China;Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
关键词：
Botrytis cinerea;broccoli;endochitinase gene;genetic transformation;Rhizoctonia solani
摘  要：
A endochitinase gene (Tch) from the fungus Trichoderma viride was introduced into broccoli (Brassica oleracea var. italica) by Agrobacterium-mediated transformation. Sixty-eight putative transformants were obtained and the presence of the Tch gene was confirmed by both PCR and Southern blot analysis. RT-PCR analysis showed an accumulation of the transcript encoding the endochitinase protein in the transgenic plants. Using real-time quantitative PCR, the expression profiling of endochitinase gene was analyzed. Primary transformants and selfed progeny were examined for expression of the endochitinase using a fluorometric assay and for their resistance to the pathogenic fungi Botrytis cinerea and Rhizoctonia solani. The endochitinase activities in T-0 in vitro plants, T-0 mature plants and T-1 mature plants were correlated with leaf lesions, and the transgenic line T618 had high endochitinse activities of 102.68, 114.53 and 120.27 nmol L-1 MU min(-1) mg(-1) protein in the three kinds of plants, respectively. The endochitinase activity showed a positive correlation with the resistance to the pathogens. Most transgenic To broccoli had increased resistance to the pathogens of B. cinerea and R. solani in leaf assays and this resistance was confirmed to be inheritable. These findings suggested that expression of the Tch gene from T viride could enhance resistance to pathogenic fungi in Brassica species.

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