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Overexpression of the peroxidase gene ZmPRX1 increases maize seedlingdrought tolerance by promoting root development and lignification

作  者:
Zhai, Xiuzhen;Yan, Xiaocui;Zenda, Tinashe;Wang, Nan;Dong, Anyi;Yang, Qian;Zhong, Yuan;Xing, Yue;Duan, Huiju
单  位:
Hebei Agr Univ, Coll Agron, State Key Lab North China Crop Improvement & Regul, Key Lab Crop Germplasm Resources North China,Minis, Baoding 071001, Hebei, Peoples R China
关键词:
Drought tolerance;Maize;ZmPRX1;Root development;Lignin biosynthesi
摘  要:
Drought is a main abiotic stress factor hindering plant growth, development, and crop productivity. Therefore, it is crucial to understand the mechanisms by which plants cope with drought stress. Here, the function of the maize peroxidase gene ZmPRX1 in drought stress tolerance was investigated by measurement of its expression in response to drought treatment both in a ZmPRX1 overexpression line and a mutant line. The higher root lignin accumulation and seedling survival rate of the overexpression line than that of the wild type or mutant support a role for ZmPRX1 in maize drought tolerance by regulating root development and lignification. Additionally, yeast one-hybrid, Dule luciferase and ChIP-qPCR assays showed that ZmPRX1 is negatively regulated by a nuclear-localized ZmWRKY86 transcription factor. The gene could potentially be used for breeding of drought-tolerant cultivars. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V.

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