单 位：
Tieling Normal College of Liaoning Province,Tieling,Liaoning 112001,Chin;Tieling Normal College of Liaoning Province,Tieling,Liaoning 112001,China
关键词：
the idea;tube seedlings;scabra;differentiation;medium;rootin
摘 要：
[Objective] The research aimed to establish the propagation system of tissue culture seedlings of Gentiana scabra Bunge.[Method] Using the tender stems of G.scabra as materials,the callus induction and differentiation,the differentiation and rooting of adventitious buds,the transplanting of tube seedlings were studied.[Result] The ideal medium for inducing the tender stems to form the differentiation calli was MS+ 0.2mg/L BA+ 1.0-1.5mg/L 2,4-D.The ideal medium for the differentiation culture of calli and adventitious buds was MS 1.2mg/L AgNO3+ 0.6mg/L BA+ 0.1mg/L NAA.The ideal medium for the rooting culture and rooting sub-culture of G.scabra tube seedling was 1/2 MS+ 0.1mg/L IAA+ 0.3mg/L NAA.The botanic characters of G.scabra tube seedlings transplanted on the hillside were remained.[Conclusion] Under these experimental conditions,the transplanting survival ratio of cultured tube seedlings reached 96%,indicating that the method can be used in field production completely.
