Nor Hafizah Zakaria;Fadzilah Adibah Abdul Majid;Anis Fadhlina;Siti Noorwanie Abdul Hamid;Mohd Nur Nasyriq Anuar;Tengku Nurfarhana Tengku Azi

Institute of Climate Adaptation and Marine Biotechnology (ICAMB), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Proliv Life Sciences Sdn Bhd, Taman Ukay Bistari, 68000 Ampang, Selangor, Malaysi; Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia; Discipline of Basic Health Sciences, Faculty of Pharmacy, Universiti Sultan Zainal Abidin, Besut Campus, 22200, Besut, Terengganu, Malaysia;Bioprocess and Polymer Engineering Department, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81030 Johor Bahru, Johor, Malaysia

pe3;italic;ic;skin;50;elastas

A natural product-based dietary approach could offer a safe and effective method for slowing down or preventing age-related deterioration in skin appearance and function, including issues like hyperpigmentation, dryness and wrinkles. Proliv Essence-3 (PE3) is a botanical beverage, meant for oral consumption with a unique formulation of three selected fruit extracts, Momordica charantia, Malus domestica, and Psidium guajava. Its purpose is to enhance both skin health and overall well-being from within. Proximate composition, presence of gallic acid and antioxidant activity of PE3 extract were determined. Elastase and tyrosinase inhibition assays were employed to investigate the anti-aging and whitening effects, respectively. The in vitro scratch assay and epidermal growth factor (EGF) assay were carried out to evaluate skin cell growth promotion and rejuvenation. The cytotoxicity analysis was carried out via neutral red uptake. The proximate analysis revealed that the product had a high moisture content and low amounts of calories. High-performance liquid chromatography (HPLC) analysis estimated 11.22 mg of gallic acid in 1 g of PE3 extract. PE3 exhibited a DPPH-IC₅₀ value of 148.60±1.52 μg/mL and an ABTS-IC₅₀ value of 91.18 ±1.15 μg/mL. The IC₅₀ values for tyrosinase and elastase inhibition assays were 160.20 ±1.81 μg/mL and 65.49±0.38 μg/mL, respectively. PE3 was also discovered to be non-cytotoxic, and it enhanced the migration and proliferation of HSF1184 cells. EGF secretion was detected in PE3-treated HSF1184. This study provided preliminary evidence supporting the potential of PE3 as a nutricosmetical botanical beverage for promoting skin beautification and general health.