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园艺学报(英文版)
2023,10
(1)
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Horticultural Plant Journal
2023,10
(1)
A 1-bp deletion in the MC04g1399 is highly associated with failure to produce fruit wart in bitter gourd (Momordica charantia L.)
作 者：
Jia Liu;Junjie Cui;Jichi Dong;Jian Zhong;Chunfeng Zhong;Fanchong Yuan;Wendong Guan;Fang Hu;Jimin Cheng;Kailin H
单 位：
College of Horticulture, South China Agricultural University/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs/Guangdong Vegetables Engineering Research Center, Guangzhou, Guangdong 510642, China; Henry Fok School of Biology and Agricultural, Shaoguan University, Shaoguan, Guangdong 512023, China;College of Horticulture, South China Agricultural University/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs/Guangdong Vegetables Engineering Research Center, Guangzhou, Guangdong 510642, Chin;Department of Horticulture, Foshan University, Foshan, Guangdong 528225, China
关键词：
Bitter gourd;Fruit wart;Bulk segregant analysis;Fine-mapping;Candidate gene;ERECTA FAMILY GENES;TRICHOME DEVELOPMENT;MOMORDICA-CHARANTIA;SPINE DENSITY;IDENTIFICATION;DNA;INITIATION;MERISTEM;REVEALS;PEPTID
摘 要：
Fruit wart is an important appearance trait influencing consumer preferences of bitter gourd. The molecular genetic mechanisms underlying fruit wart formation in bitter gourd are largely unknown. In this study, genetic analysis based on four generations showed that fruit wart formation in bitter gourd was controlled by a single dominant locus named as Fwa. The Fwa locus was initially mapped into a 4.82 Mb region on pseudochromosome 4 by BSA-seq analysis and subsequently narrowed down to a 286.30 kb region by linkage analysis. A large F2 population consisting of 2 360 individuals was used to screen recombinants, and the Fwa locus was finally fine mapped into a 22.70 kb region harboring four protein-coding genes through recombination analysis. MC04g1399, encoding an epidermal patterning factor 2-like protein, was proposed as the best candidate gene for Fwa via sequence variation and expression analysis. In addition, a 1-bp insertion and deletion (InDel) variation within MC04g1399 was converted to a cleaved amplified polymorphic sequence (CAPS) marker that could precisely distinguish between the warty and non-warty types with an accuracy rate of 100% among a wide panel of 126 bitter gourd germplasm resources. Our results not only provide a scientific basis for deciphering the molecular mechanisms underlying fruit wart formation but also provide a powerful tool for efficient genetic improvement of fruit wart via marker-assisted selection.
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A 1-bp deletion in the MC04g1399 is highly associated with failure to produce fruit wart in bitter gourd [Jia Liu, Junjie Cui, Jichi Dong, Jian Zhong, Chunfeng Zhong, Fanchong Yuan, Wendong Guan, Fang Hu, Jiaowen Cheng, Kailin H] 园艺学报(英文版) 2024,10 (1)
