中国农业期刊集成服务平台

当前位置: 首页 > 文章 > Necroptosis contributes to deoxynivalenol-induced liver injury and inflammation in weaned piglets 畜牧与生物技术杂志(英文版) 2024,15 (1)

Position: Home > Articles > Necroptosis contributes to deoxynivalenol-induced liver injury and inflammation in weaned piglets Journal of Animal Science and Biotechnology 2024,15 (1)

Necroptosis contributes to deoxynivalenol-induced liver injury and inflammation in weaned piglets

作者：

Qilong Xu;Henry H. Gong;Mohan Zhou;Junjie Guo;Shaokui Chen;Kan Xiao;Yulan Li

关键词：

Deoxynivalenol;Liver damage;Necroptosis;Necrostatin-1;Pig

摘要：

The aim of this study was to investigate the role of necroptosis in deoxynivalenol (DON)-induced liver injury and inflammation in weaned piglets.In Exp. 1, 12 weaned piglets were divided into 2 groups including pigs fed basal diet and pigs fed diet contaminated with 4 mg/kg DON for 21 d. In Exp. 2, 12 weaned piglets were divided into 2 groups including control piglets and piglets given a gavage of 2 mg/kg body weight (BW) DON. In Exp. 3, 24 weaned piglets were used in a 2 × 2 factorial design and the main factors including necrostatin-1 (Nec-1) (DMSO or 0.5 mg/kg BW Nec-1) and DON challenge (saline or 2 mg/kg BW DON gavage). On 21 d in Exp. 1, or at 6 h post DON gavage in Exp. 2 and 3, pigs were killed for blood samples and liver tissues. Liver histology, blood biochemical indicators, and liver inflammation and necroptosis signals were tested.Dietary or oral gavage with DON caused liver morphological damage in piglets. Dietary DON led to hepatocyte damage indicated by increased aspartate transaminase (AST) activity and AST/alanine aminotransferase (ALT) ratio, and DON gavage also caused hepatocyte damage and cholestasis indicated by increased AST and alkaline phosphatase (AKP) activities. Dietary DON caused liver necroptosis indicated by increased protein abundance of total receptor interacting protein kinase 3 (t-RIP3) and total mixed lineage kinase domain-like protein (t-MLKL). Moreover, DON gavage increased mRNA expression of interleukin (IL)-6 and IL-1β in liver. DON gavage also induced liver necroptosis demonstrated by increased protein abundance of t-RIP3, phosphorylated-RIP3 (p-RIP3), t-MLKL and p-MLKL. However, pretreatment with Nec-1, a specific inhibitor of necroptosis, inhibited liver necroptosis indicated by decreased protein expression of t-RIP3, p-RIP3, t-MLKL and p-MLKL. Nec-1 pretreatment reduced liver morphological damage after DON gavage. Pretreatment with Nec-1 also attenuated liver damage induced by DON indicated by decreased activities of AST and AKP. Furthermore, Nec-1 pretreatment inhibited liver mRNA expression of IL-6 and IL-1β after DON challenge.Our data demonstrate for the first time that necroptosis contributes to DON-induced liver injury and inflammation in piglets.

相似文章

Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function [Tae Hong Kang, Sang In Le] 畜牧与生物技术杂志(英文版) 2024,15 (1)
Phenethyl isothiocyanate as an anti-nutritional factor attenuates deoxynivalenol-induced IPEC-J2 cell injury through inhibiting ROS-mediated autophagy [Shuiping Liu, Xinru Mao, Ge Liu, Lili Hou, Guannan Le, Fang Gan, Lixin Wen, Kehe Huan] 动物营养（英文） 2022,8 (1)
Chlorogenic acid alleviates IPEC-J2 pyroptosis induced by deoxynivalenol by inhibiting activation of the NF-κB/NLRP3/caspase-1 pathway [Yanmei Xue, Fuchang Li, Rui Li, Xinru Zhang, Huijun Guo, Chunyang Wan] 畜牧与生物技术杂志(英文版) 2024,15 (1)
Mycotoxin binder improves growth rate in piglets associated with reduction of toll-like receptor-4 and increase of tight junction protein gene expression in gut mucosa [Linghong Jin, Wei Wang, Jeroen Degroote, Noémie Van Noten, Honglin Yan, Maryam Majdeddin, Mario Van Poucke, Luc Peelman, Anne Goderis, Kurt Van De Mierop, Ronny Mombaerts, Stefaan De Smet, Joris Michiel] 畜牧与生物技术杂志(英文版) 2017,8
Occurrence of mycotoxins in feed ingredients and complete feeds obtained from the Beijing region of China [Xiaoying Li, Lihong Zhao, Yu Fan, Jia Yang, Lei Sun, Shanshan Ma, Cheng Ji, Qin Ma, Jianyun Zhan] 畜牧与生物技术杂志(英文版) 2014,5 (4) 471-478
Intestinal toxicity of deoxynivalenol is limited by supplementation with Lactobacillus plantarum JM113 and consequentially altered gut microbiota in broiler chickens [Shengru Wu, Yanli Liu, Yongle Duan, Fangyuan Wang, Fangshen Guo, Yan Fang, Xiaojun Yang, Xin Yan] 畜牧与生物技术杂志(英文版) 2018,9
Alleviation of mycotoxin biodegradation agent on zearalenone and deoxynivalenol toxicosis in immature gilts [Donghui Shi, Jiechao Zhou, Lihong Zhao, Xiaoping Rong, Yu Fan, Humera Hamid, Wenqiang Li, Cheng Ji, Qin M] 畜牧与生物技术杂志(英文版) 2018,9
4-Phenylbutyric acid accelerates rehabilitation of barrier function in IPEC-J2 cell monolayer model [Qian Jiang, Jie Yin, Jiashun Chen, Xiaokang Ma, Miaomiao Wu, Xilong Li, Kang Yao, Bin Tan, Yulong Yi] 动物营养（英文） 2021,7 (4)
Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets [Yongsong Bai, Kaidi Ma, Jibo Li, Zhongshuai Ren, Jing Zhang, Anshan Sha] 畜牧与生物技术杂志(英文版) 2022,13 (1)
Ferroptosis is involved in deoxynivalenol-induced intestinal damage in pigs [Meng Li, Lei Zhang, Yixin Mo, Jiahuan Li, Jiacheng Yang, Juan Wang, Niel A. Karrow, Hao Wu, Luo Su] 畜牧与生物技术杂志(英文版) 2023,14 (1)

导出引用

计量

文章访问数: 12

HTML全文浏览量: 0

PDF下载量: 0

Necroptosis contributes to deoxynivalenol-induced liver injury and inflammation in weaned piglets

相似文章

所属期刊

畜牧与生物技术杂志(英文版)

推荐期刊

动物营养(英文)

畜牧与生物技术杂志(英文版)