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当前位置: 首页 > 文章 > Process research of the refined Tianma Gouteng Decoction by macroporous resin method. 药用植物研究(英文版) 2012,3 (7)
Position: Home > Articles > Process research of the refined Tianma Gouteng Decoction by macroporous resin method. Medicinal Plant 2012,3 (7)

Process research of the refined Tianma Gouteng Decoction by macroporous resin method.

作  者：
Xinli Song;Lu Wen;Feng Gu
关键词：
gastrodin;sample volume;elution;method;refined;tianma gouteng decoctio
摘  要：
[Objective] To optimize the refined Tianma Gouteng decoction by AB-8 macroporous resin.[Method] With gastrodin content and paste-forming rate as the indices,the maximum sample volume,loading rate,eluent concentration and other factors were optimized during the purification process by single factor test and orthogonal test.[Result] The average paste-forming rate was 21.252 7% before resin purification process and RSD was 1.009 6%;the average gastrodin content was 5.263 mg/g and RSD was 0.693%.The optimal process was as follows: 30 ml maximum sample volume,2 BV/h loading rate,40% ethanol as elution solvent,165 ml elution volume,0.5 g/ml liquid concentration,and 1:10 diameter-height ratio.Under this condition,the dry extract rate was 9.98% and the average content of gastrodin was 13.364 mg/g.[Conclusion] This method was reasonable and feasible,could enhance the content of gastrodin,and reduce the extracts amount.

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