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Position: Home > Articles > Genetic analysis and fine mapping of a grain size QTL in the smallgrain sterile rice line Zhuo201S Journal of Integrative Agriculture 2024,23 (7)

Genetic analysis and fine mapping of a grain size QTL in the smallgrain sterile rice line Zhuo201S

作  者:
Lei, Bin;Shao, Jiale;Zhang, Feng;Wang, Jian;Xiao, Yunhua;Cheng, Zhijun;Tang, Wenbang;Wan, Jianmin
单  位:
Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improve, Beijing 100081, Peoples R China;Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China;Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China;Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
关键词:
rice;grain size;map-based cloning
摘  要:
The development and application of the small-grain rice sterile line Zhuo201S (Z201S) has demonstrated its potential for mechanized hybrid rice seed production, leading to significant cost reductions. However, the molecular mechanism responsible for the small-grain size characteristic of Z201S remains unclear. In this study, we conducted a genetic analysis using near-isogenic lines constructed from Z210S, a small-grain rice sterile line, and R2115, a normal-grain variety. The results revealed that the small-grain trait in Z201S is governed by a single partially dominant gene which also enhances grain number. Through mapping, we localized the causal gene to the short arm of chromosome 2, within a 113 kb physical region delimited by the molecular markers S2-4-1 and LB63. Transgenic analysis and gene expression assays indicated LOC_Os02g14760 as the most likely candidate gene, suggesting that the small-grain size trait of Z201S is controlled by a novel locus that has not been previously identified.

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