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Position: Home > Articles > Characterization and mapping of a novel light-dependent lesion mimic mutant Imm6 in rice (Oryza sativa L.) Journal of Integrative Agriculture 2015,14 (9)

Characterization and mapping of a novel light-dependent lesion mimic mutant Imm6 in rice (Oryza sativa L.)

作者：

Xiao Gui-qing;Zhang Hai-wen;Lu Xiang-yang;Huang Rong-feng

单位：

Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China;Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China;Hunan Agr Bioengn Res Inst, Changsha 410128, Hunan, Peoples R China

关键词：

rice;lesion mimic mutant;gene mapping;ROS;blast resistance

摘要：

A novel rice lesion mimic mutant (LMM) was isolated from an ethane methyl sulfonate (EMS)-induced 02428 mutant bank. The mutant, tentatively designated as Imm6, develops necrotic lesions in the whole growth period along with changes in several important agronomic traits. We found that the initiation of the lesions was induced by light and cell death occurred in Imm6 accompanied with accumulation of reactive oxygen species (ROS). The lower chlorophyll content, soluble protein content and superoxide dismutase (SOD) activity, the higher nnalondialdehyde (MDA) content were detected in Imm6 than in the wild type (WT). Moreover, the observation by transmission electronic microscope (TEM) demonstrated that some organelles were damaged and the stroma lamella of chloroplast was irregular and loose in mesophyll cell of Imm6. In addition, Imm6 was more resistant than WT to rice blast fungus Magnaporthe grisea infection, which was consistent with increased expression of four genes involved in the defense-related reaction. Genetic analysis showed that mutant trait of Imm6 is inherited as a monogenic recessive nuclear gene located on the long arm of chromosome 6. Using simple sequence repeat (SSR) markers, the target gene was finally delimited to an interval of 80.8 kb between markers MM2359 and MM2370, containing 7 annotated genes. Taken together, our results provide the information to identify a new gene involved in rice lesion mimic, which will be helpful in clarifying the mechanism of cell death and disease resistance in rice.

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Characterization and mapping of a novel light-dependent lesion mimic mutant Imm6 in rice (Oryza sativa L.)

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