Position: Home > Articles > Determination of iE-DAP in rumen fluid of lactating goats by Pre-column Chiral Derivatization-HPLC
Animal Husbandry & Veterinary Medicine
2018
(6)
47-51
柱前手性衍生化-高效液相色谱法测定奶山羊瘤胃液中iE-DAP的浓度
作 者：
汪艳;张欢敏;常广军;沈向真
关键词：
iE-DAP;柱前衍生化;高效液相色谱;瘤胃液;奶山羊
摘 要：
为了研究高精料日粮模式下丁酸钠对奶山羊瘤胃液中i E-DAP含量的影响,选取10头萨能奶山羊,随机分为高精料组和丁酸钠调控组,建立一种柱前手性衍生化-高效液相色谱(HPLC)法检测γ-D-谷氨酸-内消旋-二氨基庚二酸(iE-DAP)含量,并以邻苯二甲醛(OPA)和N-乙酰基-L半胱氨酸(NAC)为衍生化试剂,以0.05 mol/mL醋酸铵-乙腈(90∶10,V∶V)为流动相,紫外检测器检测波长345 nm。结果显示:在优化的色谱条件下,iE-DAP检测浓度在5~500μg/mL范围内线性关系良好,日内、日间精密度分别为5.35%、6.50%;与高精料组相比,丁酸钠调控组奶山羊瘤胃液中iE-DAP含量极显著降低(P<0.01)。结果表明:试验建立的方法可行,可用于奶山羊瘤胃液中iE-DAP含量的测定。
译 名：
Determination of iE-DAP in rumen fluid of lactating goats by Pre-column Chiral Derivatization-HPLC
作 者：
WANG Yan;ZHANG Huanmin;CHANG Guangjun;SHEN Xiangzhen;College of Veterinary Medicine,Nanjing Agricultural University;
单 位：
WANG Yan%ZHANG Huanmin%CHANG Guangjun%SHEN Xiangzhen%College of Veterinary Medicine,Nanjing Agricultural University
关键词：
iE-DAP;;pre-column derivatization;;HPLC;;rumen fluid;;goat
摘 要：
To investigate the effect of sodium butyrate supplementation on the concentration of iE-DAP in rumen fluid of lactating goats in high-concentrate diet,10 Saanen dairy goats were chosen and randomly divided into a high-concentrate group and a sodium-butyrate-regulated group. Then,a high performance liquid chromatography( HPLC) method combined with pre-column chiral derivatization was established to detect the concentration of γ-D-glutamic acid-meso-diaminopi-melic acid( iE-DAP) in goat rumen fluid. iE-DAP was derivatized with o-phthaldehyde and N-acetyl-cysteine reagent and using a mobile phase consisting of 0. 05 mol/mL ammonium acetate-acetonitrile( 90: 10),at the detection wavelength of 345 nm. The results showed that the linear range of iE-DAP was 5-500 μg/mL,the RSDs of intra-day and inter-day were lower than 7%. Compared with the high-concentrate group,the concentration of iE-DAP in the rumen fluid of the sodium-butyrate-regulated group significantly decreased( P<0. 01). The method established here was practical and could be used to effectively detect the concentration of iE-DAP in goat rumen fluid.
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