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当前位置: 首页 > 文章 > 谷子MLO家族的全基因组鉴定和表达谱分析 核农学报 2018 (8) 1492-1501
Position: Home > Articles > Characterization and Expression Analysis of MLO Gene Family in Foxtail Millet(Setaria italica) Journal of Nuclear Agricultural Sciences 2018 (8) 1492-1501

谷子MLO家族的全基因组鉴定和表达谱分析

作  者：
刘宝玲;孙岩;郝青婷;张莉;薛金爱;贾小云;李润植
关键词：
谷子;基因家族;MLO;生物信息学;表达分析
摘  要：
MLO是高等植物特有的一类抗病家族,在响应非生物胁迫中发挥重要作用。为全面解析MLO基因在谷子基因组中的特征,本研究利用生物信息学的方法,从谷子基因组中共鉴定出12个MLO基因,并对其编码蛋白的结构特征、亚细胞定位、系统进化关系、保守氨基酸残基位点进行预测,分析MLO基因在不同组织中的表达特征。结果表明,谷子MLO基因编码的蛋白质均包含有一个MLO结构域和多个跨膜区,且大多定位于细胞膜上,并存在一些保守的氨基酸残基位点和基序;与其他物种MLO成员的聚类结果表明,谷子MLO可分为7个类群(Ⅰ~Ⅶ),其中第Ⅶ类全部由禾本科作物的MLO成员组成,这些可能是禾本科作物单独进化出的一类特有的MLO成员;Si MLO10与单子叶植物中已知的抗白粉病MLO基因聚在第Ⅳ类,是谷子中一个候选的抗白粉病基因,而单子叶和双子叶植物中已知功能的MLO成员分别聚在第Ⅳ、第Ⅴ类群,表明MLO功能的形成可能发生在单、双子叶植物分化之后。大多数MLO基因在谷子的根、茎、叶、穗中均有表达,且不同的基因表达量存在较大差异,说明谷子MLO家族成员可能具有不同的生物学功能。本研究结果为揭示谷子MLO基因的功能及其在谷子抗白粉病育种中的应用奠定了一定的理论基础。
译  名：
Characterization and Expression Analysis of MLO Gene Family in Foxtail Millet(Setaria italica)
作  者：
LIU Baoling;SUN Yan;HAO Qingting;ZHANG Li;XUE Jin'ai;JIA Xiaoyun;LI Runzhi;Institute of Molecular Agriculture & Bioenergy,Shanxi Agricultural University;
关键词：
Setaria italica;;gene family;;MLO;;bioinformatics;;gene expression
摘  要：
MLO,a plant-specific disease resistance gene family,was also found to play important roles in responses to abiotic stress. To explore their functions in foxtail genome,12 Si MLO genes were identified through bioinformatics. The characterization of protein structure,sub-cellular location,phylogeny,conservative amino acid residues and gene expression were systematically analyzed. Structural analysis revealed that these proteins have one conserved MLO domain and multiple transmembrane domains,and conserved residues and motifs are also existed in these proteins. The majority of Si MLO proteins are located in plasma membrane. Phylogenetic analysis of MLO genes from foxtail and other species divided them into seven clades(Ⅰ ~ Ⅶ). All members from clade Ⅶ specifically belong to the family of Gramineae,suggesting that there exists a separate evolution process among these genes. Si MLO10 grouped into clade Ⅳ comtaining powdery mildrew resistance genes,show this gene with potential function in disease resistance. All known functional MLO genes from monocots and dicots were classed into clades Ⅳ and Ⅴ,respectively,indicating that the divergence of monocots and dicots preceded the formation of MLO function. Foxtail millet RNA-seq data unraveled that MLO genes have distinct expression patters in different tissues,suggesting these genes probably have distinct functions. These results will lay a foundation for further understanding the evolution,functions of MLO genes,as well as breeding new foxtail millet varieties resistant to powdery mildew disease.

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