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当前位置: 首页 > 文章 > 多油辣木PKM-1花药培养获得愈伤组织 云南农业大学学报(自然科学) 2018 (2) 308-313
Position: Home > Articles > Obtaining Callus from Anther Culture of Moringa oleifera Lam. cv. PKM-1 Journal of Yunnan Agricultural University(Natural Science) 2018 (2) 308-313

多油辣木PKM-1花药培养获得愈伤组织

作  者：
黄苏南;汪秉琨;杨睿;张慧;张舒昱;董蕾;田洋;曾千春
关键词：
多油辣木;PKM-1;花药培养;愈伤组织;MS培养基
摘  要：
【目的】针对多油辣木(Moringa oleifera Lam.)PKM-1品种内高度杂合、种性容易退化的问题,拟通过花药培养获得双单倍体植株,实现品种内纯合。【方法】基本培养基MS添加激素2,4-D和NAA,诱导多油辣木PKM-1花药愈伤组织,对花药愈伤组织进行秋水仙素加倍,以及添加6-BA、KT和NAA分化植株。【结果】PKM-1花药愈伤组织形成与2,4-D密切相关,2.0 mg/L时愈伤组织平均诱导率6.0%;NAA诱导花药愈伤组织,效果不明显。秋水仙素和处理时间对辣木花药愈伤组织染色体加倍具有剂量效应,0.6%秋水仙素处理72 h和0.8%秋水仙素处理48 h获得相同加倍效率,二倍体诱导率达60%。没有添加秋水仙素的对照,也形成少量二倍体愈伤组织,说明辣木花药愈伤组织存在一定比例的自然加倍。添加不同浓度6-BA、KT和NAA,诱导辣木花药愈伤组织分化,但均未分化出胚状体。【结论】本研究初步建立了多油辣木PKM-1花药培养获得愈伤组织的技术体系,但分化完整植株有待进一步研究。
译  名：
Obtaining Callus from Anther Culture of Moringa oleifera Lam. cv. PKM-1
作  者：
HUANG Sunan;WANG Bingkun;YANG Rui;ZHANG Hui;ZHANG Shuyu;DONG Lei;TIAN Yang;ZENG Qianchun;College of Agronomy and Biotechnology, Yunnan Agricultural University;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University;Yunnan Institute of Moringa, Yunnan Agricultural University;
关键词：
Moringa oleifera;;PKM-1;;anther culture;;callus;;Murashige and Skoog media
摘  要：
[Purpose]Moringa oleifera, highly heterozygous in a variety, is the main Moringa species currently planted in China, its elite characteristics of a cultivar is likely to be regressive in the progenies. In order to realize homozygous in a variety, Moringa anther culture was exploited to obtain doubled haploid plants.[Method]The anther of Moringa PKM-1 was cultured on MS media supplemented with either 2,4-D or NAA, the callus was treated with colchicine at various concentrations and diffenent time, and the doubled haploid callus was transferred to plantlet induction media supplemented with 6-BA, KT and NAA at variable concentrations.[Result]The callus produced from PKM-1 anther cultured on MS media was correlated to 2,4-D concentration, the maximum callus induction rate was 9.0% supplemented with 2.0 mg/L 2,4-D, and the average was 6.0%. NAA had no contribution to the callus induction of anther culture. Chromosome doubled from haploid callus was likely to be dosage effect in terms of colchicine concentration and treat time, 0.6% colchicine treated 72 h shared the same rate as 0.8% colchicine treated 48 h with a double rate at 60%. Haploid callus of PKM-1 could be doubled autonomously without colchicine at a relatively low rate. No embryoid regenerated from the plantlet induction media supplemented with 6-BA, KT and NAA at various concentrations.[Conclusion]The calli could be obtained from the anther culture of Moringa PKM-1, whereas much work should be input to generate intact plantlet of PKM-1 in the future.

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