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Position: Home > Articles > A bHLH transcription factor, CsSPT, regulates high-temperature resistance in cucumber Horticultural Plant Journal 2024,10 (2)

A bHLH transcription factor, CsSPT, regulates high-temperature resistance in cucumber

作  者：
Yonggui Liang;Chenyu Yang;Fangyan Ming;Bingwei Yu;Zhihua Cheng;Yixi Wang;Zhengkun Qiu;Xiaolan Zhang;Bihao Cao;Shuangshuang Ya
单  位：
State Key Laboratories of Agrobiotechnology, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, MOE Joint Laboratory for International Cooperation in Crop Molecular Breeding, China Agricultural University, Beijing 100193, China; Corresponding authors;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, Guangdong Vegetable Engineering and Technology Research Center, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; Corresponding authors.
关键词：
Cucumber;bHLH;CsSPT;Photosynthesis;High temperatur
摘  要：
High-temperature stress threatens the growth and yield of crops. Basic helix-loop-helix (bHLH) transcription factors (TFs) have been shown to play important roles in regulating high-temperature resistance in plants. However, the bHLH TFs responsible for high-temperature tolerance in cucumbers have not been identified. We used transcriptome profiling to screen the high temperature-responsive candidate bHLH TFs in cucumber. Here, we found that the expression of 75 CsbHLH genes was altered under high-temperature stress. The expression of the CsSPT gene was induced by high temperatures in TT (Thermotolerant) cucumber plants. However, the Csspt mutant plants obtained by the CRISPR-Cas9 system showed severe thermosensitive symptoms, including wilted leaves with brown margins and reduced root density and cell activity. The Csspt mutant plants also exhibited elevated H2O2 levels and down-regulated photosystem-related genes under normal conditions. Furthermore, there were high relative electrolytic leakage (REC), malondialdehyde (MDA), glutathione (GSH), and superoxide radical (O2.-) levels in the Csspt mutant plants, with decreased Proline content after the high-temperature treatment. Transcriptome analysis showed that the photosystem and chloroplast activities in Csspt mutant plants were extremely disrupted by the high-temperature stress compared with wild-type (WT) plants. Moreover, the plant hormone signal transduction, as well as MAPK and calcium signaling pathways were activated in Csspt mutant plants under high-temperature stress. The HSF and HSP family genes shared the same upregulated expression patterns in Csspt and WT plants under high-temperature conditions. However, most bHLH, NAC, and bZIP family genes were significantly down-regulated by heat in Csspt mutant plants. Thus, these results demonstrated that CsSPT regulated the high-temperature response by recruiting photosynthesis components, signaling pathway molecules, and transcription factors. Our results provide important insights into the heat response mechanism of CsSPT in cucumber and its potential as a target for breeding heat-resistant crops.

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