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当前位置: 首页 > 文章 > 苹果愈伤组织超表达MdNAC029促进花青苷积累 园艺学报 2018 (5) 845-854
Position: Home > Articles > Overexpression of Md NAC029 Promotes Anthocyanin Accumulation in Apple Calli Acta Horticulturae Sinica 2018 (5) 845-854

苹果愈伤组织超表达MdNAC029促进花青苷积累

作  者：
安建平;宋来庆;赵玲玲;由春香;王小非;郝玉金
关键词：
苹果;NAC转录因子;MdMYB1;花青苷
摘  要：
以‘光辉’海棠(Malus spectabilis‘Guanghui’)与‘王林’苹果(Malus×domestica‘Orin’)杂交后代中分离出来的红肉苹果果实为试验材料,克隆得到1个NAC(NAM,ATAF1/2,CUC2)转录因子基因,命名为MdNAC029。该基因开放阅读框(ORF)为843 bp,编码含有280个氨基酸的蛋白。保守结构域分析显示,MdNAC029蛋白在N端包含1个保守的NAC结构域。基因表达分析显示该基因在红肉苹果果实中表达量较非红肉果实高。在‘王林’苹果愈伤组织中超表达MdNAC029,其花青苷积累显著增加,表明MdNAC029在调控花青苷积累过程中发挥重要作用。对MdMYB1启动子序列进行分析,发现其序列包含1个MdNAC029转录因子的结合位点。同时,烟草瞬时表达试验显示,MdNAC029能够激活MdMYB1基因的表达。由此推测,MdNAC029可能通过直接促进MdMYB1基因的表达,正向调节花青苷的积累。
译  名：
Overexpression of Md NAC029 Promotes Anthocyanin Accumulation in Apple Calli
作  者：
AN Jianping;SONG Laiqing;ZHAO Lingling;YOU Chunxiang;WANG Xiaofei;HAO Yujin;College of Horticulture Science and Engineering,Shandong Agricultural University State Key Laboratory of Crop Biology;Yantai Academy of Agricultural Sciences;
关键词：
apple;;NAC transcription factor;;MdMYB1;;anthocyanin
摘  要：
The apple NAC(NAM,ATAF1/2,CUC2)gene MdNAC029 was cloned by using PCR technology from the red flesh apple that was separated from the cross between‘Guanghui'(Malus spectabilis)and‘Orin'(Malus × domestica). The open reading frame(ORF)of MdNAC029 contained 843 bp,encoding a protein of 280 amino acid residues. Conserved domain analysis showed that MdNAC029 contained a conserved NAC domain. The transcriptional analysis indicated that the expression of MdNAC029 was higher in the red flesh apple than non-red flesh apple. The MdNAC029-overexpressing apple calli exhibited increased anthocyanin content,suggesting that MdNAC029 might play an important role in regulating anthocyanin accumulation. The sequence of MdMYB1 promoter region was analyzed and a putative NAC-DNA binding motif was found. Meanwhile,the transient expression assays were performed in Nicotiana benthamiana leaves. And the results showed that MdNAC029 could induce the expression of MdMYB1. It is speculated that MdNAC029 up-regulates anthocyanin accumulation by directly promoting the transcript of MdMYB1.

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