作 者：
Gao ShuJing;Aiping Liu;Dongwei Li;Zhen Ya
单 位：
Institute of Agriculture, Inner Mongolia Agriculture University, Hohhot 010019, China;Institute of Agriculture, Inner Mongolia Agriculture University, Hohhot 010019, Chin
关键词：
grasshoppers;the same;genus;species;genomic dna;polymorphis
摘 要：
[Objective] The relationship between the genetic evolution and phylogenesis of the main grasshopper species in Inner Mongolia grasslands in molecular level was studied. [Method] Random amplified polymorphic DNA (RAPD) technique was used to amplify the 80 individuals of 8 grasshoppers (4 families, 6 genera) in Acridoidea, the polymorphisms of their genomic DNA were compared. [Result] 64 specific fragments were amplified by 7 primers with the molecular weight of 300-2 000 bp. The genetic distance between 8 grasshoppers was 0.228 2-0.589 6. Band pattern showed that polymorphism was commonly existed in different genus within the same family and different species within the same genus. The results were conducted UPGMA cluster analysis according to Nei's genetic distance, the results showed that the species within the same genus first clustered together, then the species in the same family clustered together. [Conclusion]The study could provide molecular biological basis for system development and evolution research of main grasshoppers in Inner Mongolia grassland.
