当前位置: 首页 > 文章 > 萱藻中褐藻多糖硫酸酯的纯化及其组分分析 大连海洋大学学报 2018,33 (2) 239-243
Position: Home > Articles > Purification and characterization of fucoidan from brown sea weed Scytosiphon lomentaria Journal of Dalian Ocean University 2018,33 (2) 239-243

萱藻中褐藻多糖硫酸酯的纯化及其组分分析

作  者:
周晓;宋悦凡;何云海;汪秋宽;刘舒
关键词:
萱藻;褐藻多糖硫酸酯;单糖分析;核磁共振
摘  要:
以从大连沿海新鲜萱藻Scytosiphon lomentaria中提取的褐藻多糖硫酸酯为原料,采用DEAE-Sepharose Fast Flow弱阴离子交换层析的方法对萱藻褐藻多糖硫酸酯粗提取物进行分离纯化,对纯化后各组分的硫酸根、总糖含量进行了测定,并进行组分分析。结果表明:各组分的硫酸根含量分别为10.64%、10.47%、11.93%、21.13%,多糖含量分别为46.56%、55.88%、50.06%、73.65%;通过单糖分析、红外光谱法和核磁共振波谱法分析,显示F-1、F-2含有鼠李糖,F-0、F-3出现岩藻糖的C6信号峰。本研究结果为下一步开发萱藻资源、了解其功效关系提供了理论基础。
译  名:
Purification and characterization of fucoidan from brown sea weed Scytosiphon lomentaria
作  者:
ZHOU Xiao;SONG Yue-fan;HE Yun-hai;WANG Qiu-kuan;LIU Shu;College of Food Science and Engineering,National Research & Development Branch Center for Seaweed Processing ( Dalian) ,Key Laboratory of Fishery Product Processing and Utilization of Liaoning Province,Dalian Ocean University;
关键词:
Scytosiphon lomentaria;;fucoidan;;monosaccharide composition analysis;;nuclear magnetic resonance(NMR)
摘  要:
The crude fucoidan from fresh brown sea weed Scytosiphon lomentaria collected in Dalian coast was further purified by DEAE Sepharose fast flow weak anion exchange chromatography,and then four fractions,designated as F-0,F-1,F-2,and F-3,were obtained. Their sulfate contents were determined to be 10.64%,10.47%,11.93%,21.13%,and the total polysaccharide contents were analyzed to be 46.56%,55.88%,50.06%,and 73.65%,respectively. The infrared spectroscopy and nuclear magnetic resonance analysis illustrated that F-1,and F-2 contained rhamnose,and signal peaks of fucose C6 were found in F-0 and F-3.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊