Position: Home > Articles > Anti-tumor activity and mechanism of apoptosis of tumor cells induced by ruthenium complexes
Journal of Anhui Agricultural University
2016,43
(1)
56-61
钌配合物lRu(Melm)4(4mopip)]2+稳定G-四链体对抑制肺癌细胞生长机制的研究
作 者：
孙冬冬;牟志鹏;李暖;张伟伟;赵志伟;黄东辉;杨小方;汪维云
单 位：
安徽农业大学生命科学学院
关键词：
钌配合物;G一四链体;细胞凋亡;肺癌;机制
摘 要：
制备一种新型钌配合物[Ru(MeIm)4(4mopip)]计(RuMeMo),运用元素分析(C、H、N)、电喷雾电离质谱(ESI.MS)、核磁共振谱(^1HNMR)对RuMeMo进行了结构表征.紫外光谱、热变性试验和圆二色光谱研究RuMeMo与F21T的分子作用机制,发现其能诱导线性G-四链体DNA形成混合型结构并能有效稳定G-四链体.MTT法评估RuMeMo对癌细胞的抑制作用及其对正常细胞的毒性,发现其对肺癌细胞(A549)表现出高选择的抑制作用,而对正常人类肺原胚细胞(CDDP)则表现出较低的细胞毒性.本研究结果表明,RuMeMo因其稳定G-四链体DNA的能力,从而有被运用于肺癌治疗的潜质.
译 名：
Anti-tumor activity and mechanism of apoptosis of tumor cells induced by ruthenium complexes
关键词：
ruthenium complexes%G-quadruplex%apoptosis%lung cancer%mechanism
摘 要：
In this study, a new ruthenium complex, [Ru(MeIm)4(4mopip)]2+(RuMeMo), was designed, syn- thesized, and further characterized by Elemental analyses (C, H and N), ESI-MS and NMR. UV-Visible spec- troscopy (UV-Vis), fluorescent resonance energy transfer (FRET), and circular dichroism (CD) were used to re- search the molecular mechanism of actions between RuMeMo and F21T. RuMeMo showed a high inhibitory ac- tivity to A549 and a low cytotoxicity activity to CDDP via MTT analysis. All results indicated that RuMeMo may be used for the treatment of lung cancer due to its ability for stabling G-complex.
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钌配合物[Ru(MeIm)4(4mopip]2+急定G-四链体对抑制肺癌细胞生长机制的研究 [孙冬冬, 牟志鹏, 李暖, 张伟伟, 赵志伟, 黄东辉, 杨小方, 汪维云] 安徽农业大学学报 2016,43 (1) 56-61
