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当前位置: 首页 > 文章 > 美洲鲥形态特征及其两种同工酶的组织特异性分析 南方农业学报 2018 (4) 773-779
Position: Home > Articles > Morphological characteristics of Alosa sapidissima and tissue specificity of two kinds of isozymes in it Journal of Southern Agriculture 2018 (4) 773-779

美洲鲥形态特征及其两种同工酶的组织特异性分析

作  者：
张涛;周剑光;陈建武;何力
关键词：
美洲鲥;形态特征;乳酸脱氢酶(LDH);苹果酸脱氢酶(MDH);组织特异性
摘  要：
【目的】从形态特征和生化遗传层面丰富美洲鲥种质资源研究内容,同时为筛选出鉴定美洲鲥种质的生化遗传标记打下基础。【方法】通过形态测量获取美洲鲥的可数性状和可量性状,并采用聚丙烯酰胺凝胶垂直板电泳对美洲鲥6种组织(心脏、眼睛、肌肉、肝脏、肾脏和鳃)的乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)进行检测分析。【结果】美洲鲥体侧扁,呈纺锤形,背、腹缘呈浅弧形,背部灰黑色,略带蓝绿色金属光泽,体侧和腹部均为银白色;其鳍式为背鳍D.iii-13~15,胸鳍P.i-14~17,腹鳍V.i-7~9,臀鳍A.i~ii-16~20,尾鳍C.21~25。美洲鲥鳃耙近7字形,左侧第一鳃弓外侧鳃耙数24~37+47~64。LDH和MDH在美洲鲥心脏、眼睛晶状体、肌肉、肝脏、肾脏和鳃组织中的酶带数目及其活性各不相同,呈明显的组织特异性,且美洲鲥个体间也存在差异。【结论】美洲鲥同工酶表达的组织特异性受遗传基因调控,同时与各器官组织的生化代谢活动相关。实际生产中,建议选取鳃LDH作为鉴定美洲鲥种质的生化遗传标记。
译  名：
Morphological characteristics of Alosa sapidissima and tissue specificity of two kinds of isozymes in it
作  者：
ZHANG Tao;ZHOU Jian-guang;CHEN Jian-wu;HE Li;Laboratory of Quality & Safety Risk Assessment for Aquatic Products(Wuhan),Ministry of Agriculture/Freshwater Fish Germplasm Quality Supervision and Testing Center,Ministry of Agriculture/Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences;
关键词：
Alosa sapidissima;;morphological characteristics;;lactate dehydrogenase(LDH);;malate dehydrogenase(MDH);;tissue specificity
摘  要：
【Objective】This paper was to enrich the studies about Alosa sapidissima gerplasm resources from the perspectives of morphological characteristics and biochemical genetics characteristics,and lay the foundation for screening the biochemical genetic markers that could identify A. sapidissima gerplasm resources.【Method】Countable traits and measurable traits of A. sapidissima were determined by morphometry. By using vertical polyacrylamide gel electrophoresis,lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)from six tissues(heart,eye,muscle,liver,kidney and gill)of A. sapidissima were detected and analyzed.【Result】A. sapidissima had a flat fusiform body. Its back and abdomen were shallow arc. And the back were grey and dark,with slight blue-green metallic luster. Both sides of the body and the abdomen were silver white. The fin formulas of A. sapidissima included dorsal fin D.iii-13-15,pectoral fin P.i-14-17,anal fin A.i-ii-16-20,ventral fin V.i-7-9 and caudal fin C.21-25. The morphology of the gill racker was in 7-shape and the number of outer gill racker on the left first gill arch was 24-37+47-64. The isoenzyme band numbers and activity of LDH and MDH from heart,eye,muscle,liver,kidney and gill of A. sapidissima presented tissue specificity and individual variations.【Conclusion】The tissue specificity of A. sapidissima isozymes is regulated and controlled by genetic genes,meanwhile it is related to the biochemical metabolism activities of the tissues. It is suggested to choose LDH from gill as biochemical genetic markers in A. sapidissima gerplasm identification.

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